Gradient tandem mass spectrometry

Deng, Y., Wu, J., Lloyd, T.L., Chi, C.L., Olah, T.V., Unger, S.E. (2002). High-speed gradient parallel liquid chromatography/tandem mass spectrometry with fully automated sample preparation for hioanalysis 30 seconds per sample from plasma. Rapid Commun. Mass Spectrom. 16, 1116-1123. 

Hsieh Y. et al., 2001. Quantitative screening and matrix effect studies of drug discovery compounds in monkey plasma using fast-gradient liquid chromatography/tandem mass spectrometry. Rapid Commun Mass Spectrom 15 2481. 

D. M. High-throughput cytochrome p450 inhibition assays by ultrafast gradient liquid chromatography with tandem mass spectrometry using monolithic columns. Rapid Commun Mass Spectrom 2003, 17, 509-518. 

Romanyshyn, L. Tiller, P. R. Alvaro, R. Pereira, A. Hop, C. E. Ultra-fast gradient vs fast isocratic chromatography in bioanalytical quantification by liquid chromatography/ tandem mass spectrometry. Rapid Commun Mass Spectrom 2001, 15, 313-319. 

C. L. Olah, T. V. et al. High-speed gradient parallel liquid chromatography/tandem mass spectrometry with fully automated sample preparation for bioanalysis ... 

Reverse-phase columns with a gradient elution in combination with UV-Vis spectrophotometers using photodiode-array (PDA) (Fig. 1.6) and spectrofiuorimeters are common devices employed in this technique. In a lesser extent, MS, tandem mass spectrometry (MS-MS), and nano liquid chromatography-electrospray ionization-quadrupole time-of-flight tandem mass spectrometry (nanoLC-nanoESI-Q-qTOF-MS-MS) has been used as detection system. This instrumentation has been mainly used in the analysis of dyes and proteinaceous media, and in some extent, in the analysis of drying oils and terpenoid varnishes [47,48],... 

Table 2.1.2 Gradient elution of the amino acids for tandem mass spectrometry...

Hsieh, Y., Chintala, M., Mei, H., Agans, J., Brisson, J. M., Ng, K., and Korfmacher, W. A. (2001). Quantitative screening and matrix effect studies of drug discovery compounds in monkey plasma [by] using fast-gradient liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom. 15 2481-2487. 

Ayrton, J., Plumb, R., Leavens, W. J., Mallett, D., Dickins, M., and Dear, G. J. (1998c). Application of a generic fast gradient liquid chromatography tandem mass spectrometry method for the analysis of cytochrome P450 probe substrates. Rapid Commun. Mass Spectrom. 12 217-224. 

AT Aqueous environment Genesis C column (2.1 mm x 50 mm, 3 fim) Acetonitrile (gradient from 60% to 100%) and water containing 2 mM methyl amine with 0.1% acetic acid Electrospray ionization (ESI) tandem mass spectrometry [15]... 

Song and Naidong [129] analyzed omeprazole and 5-hydroxyomepra-zole in human plasma using hydrophilic interaction chromatography with tandem mass spectrometry. Omeprazole and its metabolite 5-hydroxy omeprazole and the internal standard desoxyomeprazole were extracted from 0.05 ml of human plasma using 0.5 ml of ethyl acetate in a 96-well plate. A portion (0.1 ml) of the ethyl acetate extract was diluted with 0.4 ml of acetonitrile and 10 /il was injected onto a Betasil silica column (5 cm x 3 mm, 5 /rm) and detected by atmospheric pressure ionization 3000 and 4000 with positive electrospray ionization. Mobile phase with linear gradient elution consists of acetonitrile, water, and formic acid (from 95 5 0.1 to 73.5 26.5 0.1 in 2 min). The flow-rate was 1.5 ml/min with total rim time of 2.75 min. The method was validated for a low limit of quantitation at 2.5 ng/ml for both analytes. The method was also validated for specificity, reproducibility, stability, and recovery. 

Scott, R. J. Palmer, J. Lewis, I. A. Pleasance, S. 1999. Determination of a GW cocktail of cytochrome P450 probe substrates and their metabolites in plasma and urine using automated solid phase extraction and fast gradient liquid chromatography tandem mass spectrometry. Rapid Commun. Mass Spectrom.,13,2305-2319. 

Taylor AW, Bruno RS, Frei B, Traber MG. Benefits of prolonged gradient separation for high-performance liquid 26. chromatography-tandem mass spectrometry quantitation of plasma... 

Romanyshyn, L. Tiller, PR. Alvaro, P. Pereira,A. Cornells,E.C.A. Ultra-fast Gradient vs. Fast Isocratic Chromatography in Bioanalytical Quantification by Liquid Chromatography/Tandem Mass Spectrometry, Rapid Commun. Mass Spectrom. 15, 313-319 (2001). 

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