Glycoprotein-derived

Puromycin. Puromycin (19), elaborated by S. alboniger (1—4), inhibits protein synthesis by replacing aminoacyl-tRNA at the A-site of peptidyltransferase (48,49). Photosensitive analogues of (19) have been used to label the A-site proteins of peptidyltransferase and tRNA (30). Compound (19), and its carbocycHc analogue have been used to study the accumulation of glycoprotein-derived free sialooligosaccharides, accumulation of mRNA, methylase activity, enzyme transport, rat embryo development, the acceptor site of human placental 80S ribosomes, and gene expression in mammalian cells (51—60). 

A biomolecular system of glycoproteins derived from bacterial cell envelopes that spontaneously aggregates to form crystalline arrays in the mesoscopic range is reviewed in Chapter 9. The structure and features of these S-layers that can be applied in biotechnology, membrane biomimetics, sensors, and vaccine development are discussed. 

Ricin Cytotoxic glycoprotein derived from seeds ofthe castor plant... 

Koide, T., Foster, D.C., Yoshitake, S. and Davie, E.W. (1986) Amino acid sequence of human histidine-rich glycoprotein derived from the nucleotide sequence of its cDNA. Biochemistry 25, 2220-2225. 

The appropriate protocols for diamine modification of various carbohydrate or glycoprotein derivatives may be found in the indicated sections. 

Stenham, D.R., Campbell, J.D., Sansom, M.S.P., Higgins, C.F., Kerr, I.D. and Linton, K.J. (2003) An atomic detail model for the human ATP binding cassette transporter P-glycoprotein derived from disulphide cross-linking and homology modelling. FASEB Journal, 17, 2287-2289. 

Kakehi, K., and Honda, S. (1996). Analysis of glycoproteins, glycopeptides and glycoprotein-derived oligosaccharides by high-performance capillary electrophoresis.. Chromatogr. A 720, 377-393. 

The surfactant contains four unique proteins, designated SP-A, SP-B, SP-C, and SP-D.c e The major protein (SP-A) is a sialic acid-rich glycoprotein derived from a 26-kDa peptide, which contains a short collagen-like domain.eTk Like collagen, this domain contains glycosylated hydroxyproline. 

Hardy, M. R., and Townsend, R. R. (1994). High-pH Anion-Exchange Chromatography of Glycoprotein-Derived Carbohydrates. Methods Enzymol 230 208. 

Gliadin Gliadin, a glycoprotein derived from gluten, is extracted from wheat and separated by capillary electrophoresis [34,35]. Gliadin is classified as co-5, co-1,2, a, and y- type based on its structure and electrophoretic mobility [36].The glycoprotein is water insoluble due to the presence of interpolypeptide disulfide bonds and hydrophobic interactions. A limitation on the use of gliadins is that patient sensitivity causes an autoimmune disorder called celiac disease. 

Yoshinaka, Y., Ueda, Y., and Suzuki, S. Ion-pair chromatographic separation of glycoprotein-derived oligosaccharides as their 8-aminopyrene-l,3,6-trisulfonic acid derivatives./. Chromatogr. A. 2007, 1143,83-87. 

Ricin, a toxic glycoprotein derived from the castor bean, causes hypotension and myocardial hemorrhage. The Center for Disease Control and Prevention (CDC) lists it as a category B agent due to its easy availability as a terrorist weapon. Only a limited amount of information is available... 

Human renin, a 340-amino-acid glycoprotein derived from a larger polypeptide, prorenin, is produced and secreted by the juxtaglomerular cells in the kidney. The presence of only low levels of renin in circulation made human renin difficult to obtain in quantities required for drug screens and structural studies. Therefore, the pepsin family of aspartyl proteases was used for the modeling of human renin in drug... 

Three classes of interferon (IFN) have been characterized or (alpha). (beta), and y (gamma) (sec Table 6-6). re-interferons arc glycoproteins derived from human leukocytes, p-Intcrfcrons are glycoproteins derived from fibroblasts and macrophages. They share a receptor with re-interferons, y-Interferons are glycoproteins derived from human T lympho-... 

Schmid, K., Purification and properties of an aj-glycoprotein derived from normal human plasma. Biochim. Biophys. Acta 21, 399 (1956). 

Although not discussed in this chapter, FAB MS is widely used in the characterization of glycoprotein-derived N- and O-linked sialic-acid-containing carbohydrate chains. Tj ical information can be found in refs. [311,312]. 

C NMR data of sialic acids and glycoprotein-derived sialocarbohydrates have been reviewed in ref [16]. In several sialic-acid-related investigations, e.g. synthetic studies, C NMR spectroscopy forms part of the analysis techniques, and will not be reviewed in detail. For some additional data, more directly related with the glycoprotein glycan character of this chapter, see refs. [48,63,64,103,105,152,331,332,337,340-343]. 

Infection. Vancomycin (18)is a relatively small glycoprotein derived from Nocar-dia orientalis, which is well known for its activity against Gram-positive bacteria including Streptococci, Corynebacteria Clos-tridia. Listeria, and Bacillus. 

Fig. 17.4 Simplified structure of a complex N-linked glycan and the sites of action of B-hexosaminidase. Glycoproteins are degraded by specific enzymes from both directions. Lysosomal catabolism is terminated when an A-acetylglucosamine residue is encountered by the catalytically impaired enzyme B-hexosaminidase, and thus the glycoprotein-derived oligosaccharide in the box accumulates in Sandhoff patients.

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