Glycopeptides VOLUME

Fig. 4.5. Gel-filtration column chromatography of dansylated microbubble glycopeptide surfactant on Sephadex G-25. The column (0.9 cm I.D. x 55 cm) was equilibrated with 50 mM-Na acetate buffer, pH 4.5, containing 4 M guanidine-HCl. The column was eluted with the equilibrating buffer, at a flow rate of 3.3 ml per hour. The volume per fraction was 0.7 ml, and each fraction was combined with an added 0.8 ml aliquot of the equilibrating buffer before being analyzed for fluorescence. Activation of the dansyl label was performed at 254 nm, and the emitted fluorescent light was monitored at 450 nm. (Taken from ref. 322.)...

Biotin-derivatized carbohydrates can be immobilized on a streptavidin-coated substrate through the affinity interaction of the streptavidin-biotin pair to create carbohydrate microarrays. Biotin-derivatized carbohydrates include carbohydrate hgands that are biotinylated via a short aliphatic spacer or at the peptide part of glycopeptides. Several commercially available streptavidin-coated microweU plates can be apphed when biotin-derivatized carbohydrates are available, such as the streptavidin-coated 384-well plate with a well volume 25 pi (4,18) and a streptavidin-coated 192-spot shde format (27). The first was designed to be in maximal proximity to the traditional immunochemical assay using commercial streptavidin-coated black 384-well plates. 

Glycopeptides and Glycoproteins Synthesis, Structure, and Application Volume Editor Wittmann, V. 

Efficient separations of 2-acetamido-2-deoxy-galactitol, -glucitol and -maiuiitol, and of oUgosaccharide-hexosaminitols released by reductive -elimination from glycopeptides, from other mataials, were achieved by h.p.l.c. with pulsed amperometric detection on a surprising combination a cation-exchange column with dilute NaOH as eluant. Neutral and amino-sugars eluted near the void volume. The cationic polyol surfactants 1 and their synthetic precursors were separated by ion-pair reversed-phase h.p.l.c., and the related non-ionic diamide polyol surfactants 2 were determined by reversed-phase h.p.l.c. ... 

The sialic acids are a family of ubiquitous aminosugars found in some bacteria and invertebrates, and in all vertebrate tissues. They are normally found as nonreducing termini of complex heteropolymers such as glycoproteins, glycopeptide hormones, and glycolipids. The chemistry and structural features of these compounds are described in Chapter 1 of this volume. 

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