Glycerol, solution preparation

Stock solution 6. 60 % glycerol was prepared by mixing 12 mL glycerol with 8 mL deionized water. This solution was autoclaved and stored on the bench. 

The library was prepared by automated inkjet deposition of aqueous/glycerol solutions of metal salts with appropriate viscosity (step a. Fig. 11.13). The deposition surface was composed of carbon paper to ensure electric conduction and the desired chemical inertness. The deposited salts were reduced to metallic elements (step b) ... 

Isopropylideneglycerol, a five-membered cyclic hydroxy ketal from acetone and glycerol, is prepared in 90% yield by removing the liberated water by an azeotropic distillation. In another procedure, calcium carbide is added directly to the reaction mixture as a desiccant. Acetaldehyde and benzaldehyde, unlike acetone, react with glycerol to form a mixture of the five- and six-membered cyclic hydroxy acetals. Alkoxy acetals are made by the acetalization of a,/3-olefinic aldehydes in weakly acidic solutions however, the addition of alcohol to the double bond may not go to completion. ... 

Six electrophoretic sample solutions are prepared using one of the proteins from step 6-20 for each sample. The final solution is composed of 0.01 ml 50% aqueous glycerol, 0.030 ml of the solution prepared in step 6-19, 0.005 ml of the bromophenol blue solution prepared in step 6-5, and 0.005 ml of the protein solution prepared in step 6-20. 

Standard strains of Staphylococcus aureus (IF012732) and Escherichia coli (IF03972), cultured in SCD agar, and a lO cfu/mL bacterial solution was prepared using 10% glycerol solution. The bacterial solution was diluted 10 times with a 100 mmol/L phosphate buffer (pH 7 or pH 10). Each plant extract was added to the solution, and the final concentration was 0.25%. These mixtures were incubated at 37 C for 24 h. After the incubation period, the reacted mixtures were again diluted with fresh SCD broth on a 96-well plate. These plates were then incubated at 37 C for 2 days. The turbidity (595 nm) of the broth in the plate was measured with a plate reader (Spectra image, Tecan, Austria), and the number of sterilized cells were estimated. We were able to define the antimicrobial materials that were able to sterilize over 10 cfu/mL of the bacteria used in this procedure. 

One of the most widely used methods of preparing biomolecules and other small specimens for shadowcasting is to apply an aerosol of the specimen dissolved in a glycerol solution to the surface of freshly cleaved mica (117, 118). An especially facile and reliable method is to use an artists airbrush, suitably modified to hold a microliter pipet tip filled with the specimen solution. While this method was originally proposed for extended macromolecules, it works well for a range of subjects from globular macromolecules to viruses. 

Figure 4 Comparative gel electrophoresis patterns of fresh (in aqueous glycerol solution) PST 1 Restriction Enzyme produced digest with a Ready-To-Go dried preparation, after 5-month storage at ambient temperature (see Franks et al.) ...

Hydroxyphenylarsine in aqueous sodium hydroxide reacts with an alkaline antimony solution, prepared from antimony trichloride with the addition of glycerol, to form a dark brown arsenostibmo-compouud, soluble in dilute alkali, insoluble in dilute acid. 

A series of iridium(III) complexes of the type Ir(L-L)3 have been prepared by reaction of a glycerol solution of Ir(acac)3 and L-L (2-arylpyridine or 2-aryl-l-qui-noline) in an open vessel equipped with a reflux condenser. 5 This represented an improvement on the analogous methodology using a domestic microwave oven as less ligand was required, but YIFs were only 0.82-1.2. In the case of 2-phenyl-l-quin-oline, a 10% yield of the Ir complex was obtained in 20 min, while only trace amounts were produced after 10 h of conventional reflux. Cyclometallated platinum(II) complexes of the type PtCl(L-L)L where L-L = 2-phenylpyridine, 2-(2 -thienyl)pyridine, or benzoquinoline have also been prepared but not with any enhancement in yield (YIF = 0.58-1.0). 55... 

Centrifuge the cells for 20 min at 4000 rpm and 4 °C. Decant medium. Resuspend each pellet in 5 mL 2YT medium. Pool the supernatants containing the bacteria and add 0.5 volume of 50% glycerol in H2O (v/v, sterile filtered on a 0.22 pm membrane) to obtain a final concentration of 16.5% glycerol in 2YT medium. Measure the OD oo of the final solution. Prepare 750 pL aHquots and snap freeze them on dry ice prior to store them at — 80 °C. 

Several bioactive phospholipids (91-96) have been synthesized. Putative neoplasm inhibitors (91-94) showed significant activities in the Walker carcinosarcoma 256 and leukemia L1210 assay systems [117]. The low-melting cytotoxic phospholipids with aziridine groups (95 and 96) capable of forming stable dispersions in aquatic glycerol solutions containing 1% egg lecithin were prepared [118]. 

The preparation and physical properties of oil/water microemulsions containing liquid paraffin, glycerol, water and blends of Tween 60 and Span 80 have been examined [180]. The decrease in micellar size as the surfactant/alcohol ratio was increased is similar to the situation observed with solubilized micellar solutions formed by non-ionic surfactants. Turbidity spectra methods of particle sizing have shown that an increase of temperature of preparation over the range 25 to 80° C led to a gradual decrease in the modal diameter and the half-width of the size distribution curve. Phase diagram studies on micellar solutions prepared at 70° C have indicated a pronounced dependence of the area of existence of microemulsions on the ratio of Tween to Span in the system and on the oil... 

We have seen in Section 5.2.1.1.2 how various amines in water can react with CO2 and enhance CO2 absorption. Sodium glycinate has been dissolved in a solution of glycerol to prepare a stable liquid membrane which facilitates the transport of CO2 via the following reactions in the presence of some moisture in the feed gas stream (Chen et al, 2000) ... 

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