Glycans mass spectrometry

Phosphorylation of Glycan Residues Inductively coupled plasma-mass spectrometry (ICP-MS) is the technique used for analysis of the metal content... 

Wallace A., Boyce J., and Balland A. (2002), Structural Characterization of Man-nose-6-phosphate-containing glycans by tandem mass spectrometry, IBC s Conference on The Impact of Post-Translational and Chemical Modifications on Protein Therapeutics, San Diego, CA. 

L. A. Gennaro, D. J. Harvey, and P. Vouros, Reversed-phase ion-pairing liquid chromatog-raphy/ion trap mass spectrometry for the analysis of negatively charged, derivatized glycans, Rapid Common. Mass Spectrom., 17 (2003) 1528-1534. 

J. E. Thomas-Oates and A. Dell, Fast atom bombardment mass spectrometry strategies for analyzing glycoprotein glycans, Biochem. Soc. Trans., 17 (1988) 243-245. 

M. V. Novotny, Y. Huang, and Y. Mechref, Microscale nonreductive release of O-linked glycans for subsequent analysis through MALDI mass spectrometry and capillary electrophoresis, Anal. Chem., 73 (2001) 6063-6069. 

Kolarich, D. and Altmann, F. 2000. N-glycan analysis by matrix-assisted laser desorption/ ionization mass spectrometry of electrophoretically separated nonmammalian proteins Application to peanut allergen Ara h 1 and olive pollen allergen Ole e 1. Anal Biochem 285 64-75. 

Recent detection methods for glycan array include fluorescent assay, SPR, MALDI-TOF mass spectrometry, and nanoparticle assay. Fluorescence-based measurement is the prevalent principle for detecting binding to glycan microarrays. Rhodamine [9],... 

Kelly, J., Masoud, H., Perry, M.B., Richards, J.C., Thibault, P. Separation and characterization of O-deacylated lipooligosaccharides and glycans derived from Moraxella catarrhalis using capillary electrophoresis-electrospray mass spectrometry and tandem mass spectrometry. Anal Biochem 233 (1996) 15-30. 

M. Guillard, J. Gloerich, H. J. C. T. Wessels, E. Morava, R. A. Wevers, and D. J. Lefeber, Automated measurement of permethylated serum A-glycans by MALDI-linear ion trap mass spectrometry, Carbohydr. Res., 344 (2009) 1550-1557. 

C. Sihlbom, I. D. Hard, M. E. Lidell, T. Noll, G. C. Hansson, and M. Backstrom, Localization of O-glycans in MUC1 glycoproteins using electron-capture dissociation fragmentation mass spectrometry, Glycobiology, 19 (2009) 375-381. 

Figure 20.4. Glycan structures (mimics) expressed by different microbes tliat are invoked in molecular mimicry. Tlie term like is used when glycan structure has been determined by biochonical/mass spectrometry studies and term crossi eactive is used when evidence for glycan structure is indirect and based on antibody inliibition or binding studies.

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