Glucocorticoid receptor domains

Xu M, Chakraborti PK, Garabedian MJ, Yamamoto KR, Simons SS Jr. Modular structure of glucocorticoid receptor domains is not equivalent to functional independence. Stability and activity of the steroid binding domain are controlled by sequences in separate domains. J. Biol. Chem. 1996 271 21430-21438. Chakraborti PK, Garabedian MJ, Yamamoto KR, Simons SS Jr. Creation of super glucocorticoid receptors by point mutations in the steroid binding domain. J. Biol. Chem. 1991 266 22075-22078. 

Two zinc-containing motifs in the glucocorticoid receptor form one DNA-binding domain... 

The individual domains of the two receptors both have structures similar to that of the glucocorticoid receptor, and they bind to DNA in a similar way, with their recognition helices in the major groove. The dimer contacts are, however, totally different. In the glucocorticoid receptor, which binds to a palindromic DNA sequence like the 434 repressor described in Chapter 8, the domains interact symmetrically in a head-to-head fashion equivalent... 

Hard, T., et al. Solution structure of the glucocorticoid receptor DNA-binding domain. Science 249 157-160, 1990. 

Rivers C, Levy A, Hancock J, Lightman S, Norman M. (1999) Insertion of an amino acid in the DNA-binding domain of the glucocorticoid receptor as a result of alternative splicing. J Clin Endocrinol Metab. 84, 4283-4286. 

Hurley DM, Accili D, Stratakis CA, et al. (1991) Point mutation causing a single amino acid substitution in the hormone binding domain of the glucocorticoid receptor in famUial glucocorticoid resistance. J Clin Invest. 87, 680-686. 

Wallberg, A.E., Neely, K.E., Hassan, A.H., Gustafsson, J.A., Workman, J.L., and Wright, A.P. (2000) Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor taul activation domain. Mol. Cell. Biol. 20, 2004-2013. 

Lewis, D.F.V. et al. (2002) Molecular modelling of the human glucocorticoid receptor (hGR) ligand-binding domain (LBD) by homology with the human estrogen receptor a (hERa) LBD quantitative structure-activity... 

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