Gene profiling

Lutgens E, Faber B, Schapira K, et al. Gene profiling in atherosclerosis reveals a key role for small inducible cytokines validation using a novel monocyte chemoattractant protein monoclonal antibody. Circulation 2005 111(25) 3443-3452. 

Galon, J. et al., Gene profiling reveals unknown enhancing and suppressive actions of glucocorticoids on immune cells, FASEB J., 16, 61, 2002. 

Ferdin J, Runej T, Calin GA (2010) Non-coding RNAs identification of cancer-associated microRNAs by gene profiling. Technol Cancer Res Treat 9 123-138... 

This may be complemented with ex vivo gene profiling of PBMC from patients on immunosuppressive drugs. Finally, instead of using fresh human PBMC and rodent splenocytes, cell lines such as the human Jurkat T-cell line and the mouse EL4 thymoma cell line can be used. 

Wang E, Miller ED, Ohnmacht GA et al. High-fidelity mRNA amplification for gene profiling. Nat... 

Kenzelmann M, Klaren R, Hergenhahn M et al. High-accuracy amplification of nanogram total RNA amounts for gene profiling. Genomics 2004 83 550-558. 

Bajwa, A, Horst, RL and Beckman, MJ, 2005, Gene profiling the effects of calcium deficiency versus 1,25-dihydroxyvitamin D induced hypercalcemia in rat kidney cortex, Arch Biochem Biophys 438 182-194... 

Meeuwsen S, Persoon-Deen C, Bsibsi M, Ravid JM, Noort JM (2003) Cytokme, chemokine and growth factor gene profiling of cultured human astrocytes after exposure to proinflammatory stimuli. Glia 43 243-253. 

Sauna PP, King AR, van der Stap LD, Repunte-Canonigo V (2005) Gene profiling of laser-microdissected brain regions and sub-regions. Brain Res Brain Res Pi otoc 15 66-74. 

Key Words Adrenergic function gene expression gene profiling microarray mRNA receptor transcription. 

Figure 11.3 Bacterial gene profiling assay of cadmium (a), lindane (b) and the mixture of both compounds (c). The x-axis denotes the stress gene promoters indicative for the different end-points.

The use of -omics should be considered for the search of new parameters and for the possibility of gene profiling after hapten treatment. 

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