MRNA profiling

Plitnick, L.M., Loveless, S.E., Ladies, G.S., Holsapple, M.P., Smialowicz, RJ., Woolhiser, M.R., Anderson, P.K., Smith, C., and Selgrade, M J., Cytokine mRNA profiles for isocyanates with known and unknown potential to induce respiratory sensitization, Toxicology, 207, 487, 2005. 

Plitnick, L.M., et al., Cytokine mRNA profiles for isocyanates with known and unknown potential to induce respiratory sensitization, Toxicology, 207, 487, 2005. 

Protein expression profiling (protein differential display) using microarrays is considered an important new tool for proteomic discovery. It is similar in concept and approach to the gene expression microarray for mRNA profiling. Sreekumar and Chinnaiyan (2002) describe a general approach for using the microarray to monitor protein expression in cancer and normal tissues. Here are the steps ... 

How do the expressions of these proteins correlate with the corresponding mRNA profiles The answer is that structure-related proteins are almost always better correlated with mRNA levels across the 60 cell lines. These kinds of studies may allow us to eventually unravel the mysteries surroxmding the complex relationships of transcriptional and translational events. 

Okamura In the Rep-Erba, knockout mice, you showed that behavioural rhythms are sustained without a shorter period length. Do those animals show a flat Crj mRNA profile ... 

There are four main types of trancriptome analyses utilized in yeast mRNA profiling, each with different benefits and deficiencies (Table 3). The first two methods rely on arraying complementary nucleotide information on a grid with subsequent hybridization of a sample preparation. These methods are based on scaling up Northern blot technology of complementary nucleic acid hybridization to assess multiple transcripts... 

Yap FIK, Cheung W, Murugasu B, Sim SK, Seah CC, and Jordan SC. Thi and Th2 cytokine mRNA profiles in childhood nephrotic syndrome evidence for increased IL-13 mRNA expression in relapse. J Am Soc Nephrol 1999 10 529-537. 

C. A. Altar, Comparison of microarray-based mRNA profiling technologies for identification of psychiatric disease and drug signatures, J. Neurosci. Methods 2004, 138,173-188. 

Blood tests Protein immunoassay DX, ED, SM Biopsy, other DNA DNA genetic test SM,DR mRNA profile SM... 

Animal blood tests Protein immunoassay DNA genetic test mRNA profile... 

Guo, M. Rup>e, MA. Danilevskaya, O.N. Yang, X. Hu, Z. (2003). Genome-wide mRNA profiling reveals heterochronic allelic variation and a new imprinted gene in hybrid maize endosperm. Plant Journal, VoL36, No.l, (October 2003), pp. 30-44, ISSN 0960-7412... 

Russell and his associates [1183] first cloned mouse P450 2R1 in a search for a liver microsomal vitamin D3 25-hydroxylase. The mRNA is abundant in liver and testis of mice and was also identified (mice) in kidney, brain, epididymis, skin, heart, muscle, and spleen [1183]. In humans, a similar mRNA profile was reported [1186], with the highest levels in testis, followed by pancreas, and then the tissues reported by Cheng et al. [1183], including liver. Thus, P450 2R1 mRNA is expressed in many tissues. Protein detection has not been reported. 

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