Fractosil

Figure 6. Effect of ionic strength on Rp for different polystyrene particle sizes using the porous Fractosil system (2A).

The slopes of the calibration curves for the HDC and Fractosil systems are 0.512 and 0.289, respectively. This indicates that the "resolution of the peak separation" for the Fractosil system is superior to that of HDC, since resolution is considered to be inversely proportional to the slope of the log particle diameter - AV calibration curve (26). However when peak spreading is taken into account, the actual relative resolution between particle populations is less for the Fractosil system 2k) a result which indicates that overall, for size distribution resolution, the HDC system is superior. 

Figure 7. Comparison of polystyrene calibration curves for HDC and porous Fractosil systems (eluant ionic strength, 7.29/wM AM A (O) HDC ( 2) Fractosil)...

Figure 15. Experimental (—) and calculated (+) chromatogram for the bimodal system at 220-nm wavelength for the Fractosil system...

An adjustable glass column of 9mm ID, packed with 5g of Fractosil 200 was connected to a Spectra Physics pump connected to solvent reservoirs with a six way rotary switch. The sample was injected onto the column with a rotary loop sampler. The effluent from the column was collected with an Isco fraction collector set so that 10 ml of eluate was collected for each... 

The following matrices were fortified for the evaluation studies of Fractosil 200 tomatoes, carrots, green apples, animal fat, milk and greenhouse soil. 

Step gradient for testing Fractosil 200 in conjunction with the Autoprep 1001. ... 

The use of the Fractosil 200 produced a flat almost noise free baseline with the flame photometric detector. 

The following chromatograms were obtained with the Fractosil 200 silica gel and the Pye moving wire detector as shown in Figure 2. They were plotted by the computer. The axes are the response in millivolts versus time in seconds. When the computer reconstructed the chromatogram, the Y-axis could be magnified from 0 to 520 millivolts. 

Experience has shown that the Fractosil 200 silica gel is not very effective for removing fat interferences when lipophilic compounds are to be analyzed. However, it s very efficient when polar compounds are to be isolated. 

FIGURE 34 Effect of the flow rate, feed concentration, and temperature on the experimental breakthrough curve for the adsorption of human serum albumin to Cibracron Blue F3G-A immobilized onto Fractosil 1000 against the amount of protein applied to the column. 

The effects of a washing step on the position and shape of a breakthrough curve and the amount of protein retained in the column are shown in Fig. 37 using hen egg white lysozyme and Cibacron Blue F3GA immobilized Fractosil 1000 system as the exemplar. In Fig. 37a the time-concentration... 

FIGURE 37 The upper panel (a) shows the time concentration curves for the loading of a HPLC-BMC column (Cibacron Blue F3GA Fractosil 1000 with HEWL) to an effluent concentrations of 2, 20, and 99% of the influent concentration, respectively, followed by washing, (b) The lower panel shows the concentration profiles in the solid phase corresponding to the numbered times the upper for this protein-sorbent combination. Data adapted from Ref. 8. 

FIGURE 38 Operational regions at 94% yield for four different utilizations of the column capacity (50, 60, 70, and 80%) levels for the loading of a HPLC-BMC column (Cibacron Blue F3GA Fractosil 1000 with HEWL). Data from Mao, Q. M. and Hearn, M. T. W 1996. Biotechnol. Bioeng., 52, 202. Reprinted by permission of Wiley-Liss, Inc., a subsidiary of John Wiley Sons, Inc. 

FIGURE 43 Comparison of the shape and position of the breakthrough curves in terms of time or the amount of protein applied and generated for the corresponding packed and fluidized columns for the adsorption of HEWL (a) and (b), respectively, and HSA (c) and (d), respectively, to Cibacron Blue F3G-A immobilized onto Fractosil 1000. The superficial velocities applied were 0.64 cm/min in (a) and (b) and 3.84 cm / min in (c) and (d), respectively. 

Oligo-a-deoxyribonucleotides have been prepared on Fractosil 500 using cyanoethylphosphoramidite chemistry. 92 a coupling efficiency of 96.5 % was estimated. 2-Methoxy-6-chloro-9-(5-hydroxypentyl)aminoacridine has been... 

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