Cibacron Blue

Fig. 17.7. Fluidised bed adsorption of G3PDH from milled yeast homogenate onto zirconia-silica Cibacron Blue. The feedstock (20% w/v) was fed to the bead mill at a rate 4.05 dm3-h 1, which corresponded to a linear flow velocity of 250 cm-h 1 within the BRG contactor with a settled bed height of 21 cm. The disrupted baker s yeast homogenate from the bead mill was applied to the integrated fluidised bed directly and terminated when C/Ca = 0.65.

Cibacron Blue 3GA, for dye-ligand affinity chromatography, 6 402 Cibacron C dyes, 9 475 Ciba Specialty Chemicals, 19 448 Cichlids, world aquaculture production in 1996, 3 186t... 

Cibacron Blue F-R (Ciba Cl Reactive Blue 182) with a monofunctional aminofluorotriazine group. 

Cibacron Blue C-R (Ciba) with a synchronised bifunctional aminofluorotriazine -sulphatoethylsulphone system. 

RP-HPLC was also employed for the investigation of the hydrolysis behaviour of two fluorotriazine reactive dyes Cibacron blue F-R and Cibacron yellow F-4G. Chromatographic measurements were performed in an ODS column (50 X 4.6 mm i.d. particle size 5 /jm) at room temperature. The mobile phase was ACN-0.05 M ammonium acetate buffer containing 1 mM acetyhrimethylammonium bromide (47 53, v/v). Flow rates were 0.8 and 0.6 ml/min depending on the dyes to be separated. Dyes were detected at 275 nm. The hydrolysis of dyes was investigated both in the absence and presence of... 

Fig. 3.124. Chromatograms of a 1 1 dye mixture (a) 40 min before addition of Na2C03, (b) 10 min after addition of Na2C03, and (c) 60 min after addition of Na2C03 flow rate = 0.6 ml/min, other conditions are described in the text. The peak at 4.57 min in (a) and the same peak (smaller in size) at 4.54 min in (b) are both attributed to the functional group of cibacron yellow ( — F) the peak at 7.54 min in (a) and the same peak (smaller in size) at 7.40 min in (b) are both attributed to the hydrolysed part of the functional group of cibacron yellow the peaks at 5.07 min in (b) and 5.22 min in (c) are attributed to the hydrolysed part of the functional group of cibacron blue. Reprinted with permission from A. Zotou et al. [175].

Fig. 5 2D PAGE of CSF before and after depletion with Cibacron blue/protein G affinity resins...

Lascu, I. Due, M. Cristea, A. Rapid large-scale purification of pig heart nucleoside diphosphate kinase by affinity chromatography on Cibacron Blue 3G-A Sepharose. Anal. Biochem., 113, 207-211 (1981)... 

C. Morgan and N. Moir,/. Chem. Educ. 73, 1040-1041 (1996). Rapid Microscale Isolation and Purification of Yeast Alcohol Dehydrogenase Using Cibacron Blue Affinity Chromatography. ... 

TosoHass Tresyl toyopearl Heparin, cibacron blue, iminodiacetate... 

Fig. 3 Schematic illustrations of SMA surface conformations. Cibacron Blue [Ciba]-functionalized MPEO derivatives as SMA on A PEU-air and C PEU-water interfaces and stearic group [Cis]-functionalized MPEO derivatives as SMA on B PEU-air and D PEU-water interfaces. Legend for representing symbols applied in schemes is listed as shown in Fig. 1 [77]. Reproduced from [173]...

The effects of a washing step on the position and shape of a breakthrough curve and the amount of protein retained in the column are shown in Fig. 37 using hen egg white lysozyme and Cibacron Blue F3GA immobilized Fractosil 1000 system as the exemplar. In Fig. 37a the time-concentration... 

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