Receptor formyl peptide

While none of these neutrophil receptors has yet been sequenced, partial purification of the formyl peptide receptors has been reported (16). The formyl peptide receptor is particularly attractive because of the extensive structure-activity studies on peptide ligands by Freer and coworkers (17,18). The receptor is a trans-membrane glycoprotein with apparent molecular weight of 60,000 based on proteolysis (19) and photoaffinity (20) labeling studies. 

Shen W, Li B, Wetzel MA, Rogers TJ, Henderson EE, Su SB, Gong W, Le Y, Sargeant R, Dimitrov DS, Oppenheim JJ, Wang JM (2000) Down-regulation of the chemokine receptor CCR5 by activation of chemotactic formyl peptide receptor in human monocytes. Blood 96 2887-2894... 

Reported applications of SASD involve modification of lipopolysaccharide (LPS) molecules and studying their interaction with albumin and an antibody directed against LPS (Wollenweber and Morrison, 1985), identification of the murine interleukin-3 receptor and an N-formyl peptide receptor (Sorenson et al., 1986), crosslinking of factor V and Va to iodinated peptides... 

Schmitt, M., Painter, R.G., Jesaitis, A.J., Preissner, K., Sklar, L.A., and Cochrane, C.G. (1983) Photoaffinity labeling of the N-formyl peptide receptor binding site of intact human polymorphonuclear leukocytes./. Biol. Chem. 258, 649-654. 

Boulay, F., Tardif, M., Brouchon, L., Vignais, P. (1990a). Synthesis and use of a novel /V-formyl peptide derivative to isolate a human /V-formyl peptide receptor cDNA. Biochem. Biophys. Res. Commun. 168, 1103-9. 

Maestes, D. C., Potter, R. M., and Prossnitz, E. R. (1999) Differential phosphorylation paradigms dictate desensitization and internalization of the N-formyl peptide receptor. J. Biol. Chem. 274, 29791-29795. 

Sklar, L. A., Sayre, J., McNeil, V. M., and Finney, D. A. (1985). Competitive binding kinetics in ligand-receptor-competitor systems. Rate parameters for unlabeled ligands for the formyl peptide receptor. Mol. Pharmacol. 28, 323-330. 

Ernst, S., Lange. C., Wilbers, A., Goebeler, V., Gerke, V., and U. Rescher, 2004, An annexin 1 N-terminal peptide activates leukocytes by triggering different members of die formyl peptide receptor family. J Immunol. 172(12) 7669-76. 

FPR HABA Hacac Hbsb Hbsn Hbt Hbtth HC = C - C6H4 - NCS-p HC = C-C6H4-NH2-p Formyl peptide receptor 2-(4 -Hydroxyazobenzene)benzoic acid Acetylacetone 2-[(l,l -Biphenyl)-4-yl]benzothiazole 2-( 1-Naphthyl)benzothiazole 2-Phenylbenzothiazole 2-(2-Thienyl)benzothiazole 4-Isothiocyanatophenylacetylene 4-Aminophenylacetylene... 

Gripentrog, J. M., Jesaitis, A. J., and Miettinen, H. M. (2000). A single amino acid substitution (N297A) in the conserved NPXXY sequence of the human N-formyl peptide receptor results in inhibition of desensitization and endocytosis, and a dose-dependent shift in p42/44 mitogen-activated protein kinase activation and chemotaxis. Biochem. J. 352(Pt 2), 399-407. 

Miettinen, H. M., Gripentrog, J. M., Mason, M. M., andjesaitis, A. J. (1999). Identification of putative sites of interaction between the human formyl peptide receptor and G protein./. Biol. Chem. 274, 27934—27942. 

Murphy, P. M., Tiffany, H. L., McDermott, D., and Ahuja, S. K. (1993). Sequence and organization of the human N-formyl peptide receptor-encoding gene. Gene 133, 285-290. 

---