Formyl peptide receptor mutants

Seifert, R., and Wenzel-Seifert, K. (2001a). Defective Gi protein coupling in two formyl peptide receptor mutants associated with localized juvenile periodontitis. J. Biol. Chem. 276, 42043-42049. 

Mills,J. S., Miettinen, H. M., Cummings, D., andjesaitis, A. J. (2000). Characterization of the binding site on the formyl peptide receptor using three receptor mutants and analogs of Met-Leu-Phe and Met-Met-Trp-Leu-Leu. /. Biol. Chem. 275, 39012-39017. 

The normal internalization of the wild-type receptor, defined as a loss of cell surface receptors (measured by decreased maximal binding or B ), was unaffected for the desensitization-deficient Thr mutant (see Fig. 6.4C,D) but may have been affected when distal carboxyl terminal residues were mutated (see Fig. 6.3). Therefore some, although not all, GPCRs show radical dissociation between desensitization and internalization. This is found not only in the dopamine Dj receptor (122) but also in the N-formyl peptide (134), the CBl cannabinoid (17), and the M2 muscarinic (155) receptors. 

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