Food analysis applications, capillary

Capillary electrophoresis (CE) is a modem analytical technique that allows the rapid and efficient separation of sample components based on differences in their electrophoretic mobilities as they migrate or move through narrow bore capillary tubes (Frazier et al., 2000a). While widely accepted in the pharmaceutical industry, the uptake of CE by food analysts has been slow due to the lack of literature dedicated to its application in food analysis and the absence of well-validated analytical procedures applicable to a broad range of food products. 

The first section of the book explores emerging novel aspects of HPLC and related separation methods based on the differential velocity of analytes in a liquid medium under the action of either an electric field (capillary electromigration techniques) or a gravitational field (field-flow fractionation). The section focusing on applications highlights four significant areas in which HPLC is successfully employed chiral pharmaceutical, environmental analysis, food analysis, and forensic science. 

Frazier, R. A. and Papadopoulou, A. (2003). Recent advances in the application of capillary electrophoresis for food analysis. Electrophoresis 24,4095 105. 

Simo, C., Elvira, C., Gonzalez, N., San Roman, J., Barbas, C. and Cifuentes, A. (2004) Capillary electrophoresis-mass spectrometry of basic proteins using a new physically adsorbed polymer coating. Some applications in food analysis, Electrophoresis, 25, 2056-2064. 

The CEC-NMR contour plot indicated that all compounds are base-lined separated resulting in distinct NMR rows in the 2D display. This example shows the high application power of CE and CEC-NMR for providing unambiguous information about substances in complex organic molecules. The first steps towards a high-throughput separation system have already been made. For the successful performance of real-world applications, NMR sensitivity must be improved. NMR probes with 1-ng sensitivity should facilitate the application of capillary separations for metabolite structure elucidation and functional food analysis. 

Frazier RA, Ames JM, and Nursten HE (1999) The development and application of capillary electrophoresis methods for food analysis. Electrophoresis 20 3156-3180. 

See also Capillary Electrophoresis Food Chemistry Applications. Chiroptical Analysis. Enzymes Immobilized Enzymes Enzyme-Based Electrodes. Extraction Solvent Extraction Principles. Flow Injection Analysis Principles. lon-SelectIve Electrodes Food Applications. Liquid Chromatography Food Applications. Spectrophotometry Organic Compounds. 

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