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Fluorescence labelled polyelectrolyte

Mizusaki M, Morishima Y, Yoshida K, Dubin PL. Interactions of micelles with fluorescence-labeled polyelectrolytes. Colloid Surfaces 1999 147 149-159. [Pg.790]

Near-field scanning optical microscopy was applied to study the effect of a 2D array of silver nanoparticles on the spatial distribution and the magnitude of the fluorescence signal enhancement for a monolayer of Rhodamine 6G (Rh6G) and fluorescently labeled polyelectrolyte PAH-F1TC. The results demonstrate inhomogeneous distribution of the fluorescence signal on the surface. [Pg.169]

In this work we employed near-field scanning optical microscopy (NSOM) to study spatially resolved optical properties of a monolayer of silver nanoparticles and their effect on the fluorescence signal of Rh6G dye and fluorescently labeled polyelectrolyte PAH-F1TC. Results of this work indicate that up to 30 times fluorescence enhancement can be achieved in small clusters with average lateral dimension between 100 and 150 nm depending on the excitation wavelength. [Pg.169]

We applied NSOM to probe the spatial distribution of the fluorescence signal from a fluorescently labeled polyelectrolyte Poly(fluorescein isothiocyanate allylamine hydrochloride) (PAH-FITC) on the surface of Ag nanoparticles. Fig. 2 images correspond to the topography (a), NSOM transmission at 488 nm (b) and NSOM fluorescence measured in the 500-520 nm spectral range (c) of a sample consisting of a monolayer of Ag nanoparticles coated with 20 PE layers and a monolayer of PAH-FITC. [Pg.170]

The differences between linearly and exponentially growing films have been explained by the inward and outward diffusion of polyelectrolytes [27, 32] hence, it depends on the presence of a mobile polymeric component. When vertical diffusion of polyelectrolytes within a PEM was observed [33], a model was proposed based on this inward and outward diffusion throughout the film of at least one of the polyelectrolytes [28]. In these studies, fluorescently labelled polyelectrolytes were used and their diffusion through the polyelectrolyte multilayer was probed using confocal microscopy. [Pg.144]

Costa T, Seixas de Melo J, Migeul MD, Lindman B, Schillen K (2009) Complex formation between a fluorescently-labeled polyelectrolyte and a triblock copolymer. J Phys Chem B 113 6205-6214... [Pg.252]

Excited State Lifetime Measurements The sensitivity of the fluorescence lifetime (rf) of a fluorescent label or probe to its environment can often yield important information concerning the conformation of a polyelectrolyte in aqueous solution [ 12,19-22,26,46,60,61,87-89]. [Pg.47]

As far as deactivation by T1+ is concerned, a fluorescent label attached to PMAA is considered [95,96] to undergo a mixture of static and dynamic quenching. (Static quenching [1] can be defined as a process that occurs too fast to resolve within the timescale of the experiment. In other words, a ground-state interaction or complex forms between the quencher and the fluorophore before excitation. Such a situation would perhaps be not unexpected when counterions condense in high concentrations to a polyelectrolyte backbone in close proximity to a fluorescent label.)... [Pg.54]

Side chain chiral polyn rs (34, in Fig. 24) have been produced via active ester synthesis, by using soluble and crosslinked samjdes of copoly(AOTcp-styrene) (unpublished work). The resulting chiral polymers are of considerable interest in separation technology, and as auxiliary n dia for asymmetric synthesis. Fluorescent labeled polymers, polyelectrolytes and hydrophobically modified ionomers (e.g. and 36), are also readily available via active ester synthesis. [Pg.36]

A further step towards efficient biomedical application of PU/PUR nanocapsules was shown by the work of Paiphansiri et al. [190]. Carboxy- and amino-functionalization of the nanocapsules surface can be introduced and tailored by an in situ carboxymethylation reaction or by physical adsorption of a cationic polyelectrolyte, i.e., poly(aminoethyl methacrylate hydrochloride) or poly(ethylene imine) (see Fig. 21). Encapsulation of an aqueous solution of suforhodamine adds a fluorescent label for fluorescence microscopic detection (see Fig. 21). Whereas the carboxy-functionalized nanocapsules do not lead to a good uptake into cells, the increased uptake of amino-functionalized fluorescent nanocapsules by HeLa cells clearly demonstrates the potential of the functionalized nanocapsules to be successfully exploited as biocarriers. These results are in good agreement with the data obtained from experiments with PS particles [192]. [Pg.35]

Similar assays for measuring protease activity were also reported by Whitten and coworkers [49], These assays utilize polystyrene microspheres that are coated with streptavidin (a biotin-binding protein) and a biotinylated anionic fluorescent conjugated polyelectrolyte or a cationic polyelectrolyte, A biotinylated quencher-labeled peptide serves as a substrate for the enzyme and when mixed with the microspheres, a strong complex similar to the one in the DNA assays described in Section 13,3,1, through the biotin-avidin interaction is created [49], A schematic drawing of the assay is shown in Figure 13,7,... [Pg.1545]

It was shown that polyelectrolyte microcapsules containing metallic nanoparticles in their walls could be remotely activated inside living cells to release encapsulated material inside them (Figure 3.4.). These metal nanoparticles served as absorption centers for the energy supplied by a laser beam. These absorption centers caused local heating that disrupted the polyelecrolyte shell and allowed the encapsulated material to leave the interior of the capsule. Fluorescently labeled polymers were used as a model system of encapsulated material. [Pg.148]

Figure 11.12 Self-reporting pressure sensing surface based on PMETAC polyelectrolyte brushes and carbo)grfluorescein. (a) Experimental design to determine the fluorescence-based output upon mechanical compression of functionalised polyelectrolyte brushes using a soft colloidal probe, (b) Fluorescence images obtained from a compression experiment on fluorescently labelled PMETAC in water i-iii, during approach, with loads of 0.7, 2.8, and 5.6 pN iv-vi, during retraction, with loads of 2.8, 0.7, and -1.4 pN. Scale bar 5 pm. Figure 11.12 Self-reporting pressure sensing surface based on PMETAC polyelectrolyte brushes and carbo)grfluorescein. (a) Experimental design to determine the fluorescence-based output upon mechanical compression of functionalised polyelectrolyte brushes using a soft colloidal probe, (b) Fluorescence images obtained from a compression experiment on fluorescently labelled PMETAC in water i-iii, during approach, with loads of 0.7, 2.8, and 5.6 pN iv-vi, during retraction, with loads of 2.8, 0.7, and -1.4 pN. Scale bar 5 pm.
Kosovan P, Limpouchova Z, Prochazka K (2006) Molecular dynamics simulation of time-resolved fluorescence anisotropy decays liom labeled polyelectrolyte chains. Macromolecules 39(9) 3458-3465. doi 10.1021/ma052557a... [Pg.146]

Fujita and coworkers [79] smdied fluorescently labeled polyoxyethylene chains and found a good correlation between the concentration dependence of the friction coefficient evaluated from the anisotropy measurements and from the macroscopic viscosity. Fujita developed the fi ee-volume theory which describes reasonably well the concentration dependence of in the whole concentration region, [80] but it does not enable prediction of the parameters at a molecular level. Hyde et al. [81] used the Fujita theory for fairly successful interpretation of the experimental data. An interesting paper has been published by Viovy and Moimerie [82]. The authors studied concentrated solutions of anthracene-labeled polystyrene in toluene. They found good correlation of the local dynamics with the viscosity in the range of high concentrations and made one very important observation the local dynamics are unaffected by the overlap of the polymer chains that occurs at concentrations higher than c (concentration of the first overlap—see chapter Conformational and Dynamic Behavior of Polymer and Polyelectrolyte Chains in Dilute Solutions ). [Pg.165]

Fig. 8 FCS proves that the two differently labeled polyelectrolytes are anchored to the same PNIPAM microgel and, thus, that the layer-by-layer assembly has been successful. Top Auto- and cross-correlation function of the coated PNIPAM nanoparticles. Bottom Confocal fluorescence images of dried particles when excited at 470 nm (left) and 532 nm (right), respectively (adapted with permission from the Journal of Physical Oiemistry [137]. Copyright (2007) American Chemical Society)... Fig. 8 FCS proves that the two differently labeled polyelectrolytes are anchored to the same PNIPAM microgel and, thus, that the layer-by-layer assembly has been successful. Top Auto- and cross-correlation function of the coated PNIPAM nanoparticles. Bottom Confocal fluorescence images of dried particles when excited at 470 nm (left) and 532 nm (right), respectively (adapted with permission from the Journal of Physical Oiemistry [137]. Copyright (2007) American Chemical Society)...
Woo F1Y, Vak D, Korystov D, Mikhailovsky A, Bazan GC, Kim DY (2007) Cationic conjugated polyelectrolytes with molecular spacers for efficient fluorescence energy transfer to dye-labeled DNA. Adv Funct Mater 17 290-295... [Pg.451]

Liu B, Bazan GC (2007) Tetrahydrofuran activates fluorescence resonant energy transfer from a cationic conjugated polyelectrolyte to fluorescein-labeled DNA in aqueous media. Chem Asian J 2 499-504... [Pg.452]

Mizusaki M., Morishima Y., Dubin P. L. Interaction of pyrene-labeled hydro-phobically modified polyelectrolytes with oppositely charged mixed micelles studied by fluorescence quenching. J. Phys. Chem. B 1998 102 1908-1915. [Pg.736]


See other pages where Fluorescence labelled polyelectrolyte is mentioned: [Pg.387]    [Pg.95]    [Pg.387]    [Pg.95]    [Pg.80]    [Pg.265]    [Pg.24]    [Pg.190]    [Pg.747]    [Pg.71]    [Pg.80]    [Pg.79]    [Pg.224]    [Pg.727]    [Pg.665]    [Pg.304]    [Pg.142]    [Pg.78]    [Pg.525]    [Pg.301]    [Pg.382]    [Pg.248]    [Pg.25]    [Pg.373]    [Pg.354]    [Pg.355]    [Pg.692]    [Pg.768]    [Pg.36]   


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Fluorescent labeling

Fluorescent labelling

Fluorescent labels

Fluorescently-labeled

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