Fluorescence labelled polyelectrolyte molecules

A similar technique, using fluorescent polymer quenching for DNA sensing, has also been reported [37,39]. This technique is more complex compared with the one described above and requires the use of other molecules than a conjugated polyelectrolyte and a quencher-labeled DNA or PNA molecule. The... 

FIGURE 2.1.8 (a) Design of virus-like polymer particles by self-assembling amphiphilic block copolymer molecules around RNA-based polyelectrolyte complexes, (b) Uptake and intracellular distribution of bilayer siRNA-polyethylenimine (PEI) complexes prepared at different concentrations of copolymer into HeLa cells after 24 h of incubation ([siRNA] = 50 nM). Copolymer and siRNA molecules are labeled with fluorescein and Cy5, respectively. Last column Overlay of DIC (differential interference contrast) and fluorescence images (blue nuclei stained with DAPI [4, 6-diamidino-2-phenylindoleD [108]. 

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