Fluorescence in situ hybridisation

Wagner, M. Horn, M. Daims, H. Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes. Curr. Opin. Microbiol. 2003, 6, 302-309. 

Saez A, Andreu FJ, Segui MA, Bare ML, Fernandez S, Dinares C, et al. HER-2 gene amplification by chromogenic in situ hybridisation (CISH) compared with fluorescence in situ hybridisation (FISH) in breast cancer-A study of two hundred cases. Breast 2006 15(4) 519-27. 

R. Erhadt, D. Bradford, R. J. Sevior, R. Amann and L. L. Blackall (1997). Development and use of fluorescent in situ hybridisation probes for the detection and identification of Microtrix parvicella in activated sludge. Syst. Appl. Microbiol., 20, 310-318. 

The spectral karyotype fluorescence in situ hybridisation (FISH)... 

Eller G., Stubner S., and Erenzel P. (2001) Group-specific 16S rRNA targeted probes for the detection of type 1 and type 11 methanotrophs by fluorescence in situ hybridisation. FEMS Microbiol. Lett. 198, 91-97. 

Taxonomic probes, including antibodies and nucleic acid or peptide nucleic acid (PNA) probes selectively stain particular target cells by association with antigens or DNA/RNA. The corresponding approaches are designated as immunofluorescence (IF) and fluorescence in situ hybridisation (FISH), respectively. The major limitation of these labelling procedures is the low fluorescence intensity, which results in poorly labelled cells that escape detection. 

Fig. 8.7 Fluorescence in situ Hybridisation (FISH) overlay image of microbial aggregate involved in anaerobic methane oxidation. Different microbes were stained using different oligonucleotide probes. Core is ANNM-2 Archaea, surrounded by sulphate-reducing Desulfosarcina imaged by laser scanning confocal microscopy. From V.J. Orphan etal. in Proc. Natl. Acad. Set USA, Vol. 99, 7663—7668, 2002. Reproduced with permission. Copyright National Academy of Sciences (2002).

Yaziji H, Barry TS, Goldstein LC, et al. Discrepancies between immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH) for HER-2 testing in breast cancer the role of quality assurance program on a cohort of 3564 patients. ASCO annual meeting proceedings. J Clin Oncol 2004, 22 (abstract 679) 14S. 

Oberheitmann B, Schafer J, Dally H, Garms A, Frentzel-Beyme R and Hoffmann W (1999) The chromosome-based challenge assay using fluorescence in situ hybridisation a possible test for increased cancer susceptibility. Mutat Res 428 157 -164. 

To measure radiation, physical sensors have been most widely utilised for biology studies so far. Recently, studies on chromosome aberrations in the peripheral lymphocytes by Fluorescence in situ hybridisation and assays of micronuclei have been developed. ... 

Finocchiario et al. cloned and sequenced a cDNA encoding human liver carnitine palmitoyltransferase in 1991 after moping it to chromosome lp32 by fluorescence in situ hybridisation. Two years later the stracture of the gene was completely resolved. It contains an open reading frame of 1974 basepairs that encodes a protein of 658 amino acid... 

Xufre, A., Albergaria, H., Inacio, J., Spencer-Martins, L, Girio, F. (2006). Application of fluorescence in situ hybridisation (FISH) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations. International Journal of Food Microbiology, 108, 376-384. 

WEBSTER N, BOURNE D and HALL M R (2006) Vibrionaceae infection in phyllosomas of the tropical rock lobster Panulirus ornatus as detected by fluorescence in situ hybridisation , A(j MacM/fMre, 255,173-178. 

Amann R, Fuchs BM, Behrens S. The identification of microorganisms by fluorescence in situ hybridisation. Curr Opin Biotechnol. 2001 12 231-236. 

Tuohy KM, Finlay RK, Wynne AG, Gibson GR. A human volunteer study on the prebiotic effects of HP-Inulin — faecal bacteria enumerated using fluorescent in situ hybridisation (FISH). Anaerobe. 2001 7 113-118. 

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