Fluorescence in situ hybridisation FISH

Saez A, Andreu FJ, Segui MA, Bare ML, Fernandez S, Dinares C, et al. HER-2 gene amplification by chromogenic in situ hybridisation (CISH) compared with fluorescence in situ hybridisation (FISH) in breast cancer-A study of two hundred cases. Breast 2006 15(4) 519-27. 

The spectral karyotype fluorescence in situ hybridisation (FISH)... 

Taxonomic probes, including antibodies and nucleic acid or peptide nucleic acid (PNA) probes selectively stain particular target cells by association with antigens or DNA/RNA. The corresponding approaches are designated as immunofluorescence (IF) and fluorescence in situ hybridisation (FISH), respectively. The major limitation of these labelling procedures is the low fluorescence intensity, which results in poorly labelled cells that escape detection. 

Fig. 8.7 Fluorescence in situ Hybridisation (FISH) overlay image of microbial aggregate involved in anaerobic methane oxidation. Different microbes were stained using different oligonucleotide probes. Core is ANNM-2 Archaea, surrounded by sulphate-reducing Desulfosarcina imaged by laser scanning confocal microscopy. From V.J. Orphan etal. in Proc. Natl. Acad. Set USA, Vol. 99, 7663—7668, 2002. Reproduced with permission. Copyright National Academy of Sciences (2002).

Yaziji H, Barry TS, Goldstein LC, et al. Discrepancies between immunohistochemistry (IHC) and fluorescence in situ hybridisation (FISH) for HER-2 testing in breast cancer the role of quality assurance program on a cohort of 3564 patients. ASCO annual meeting proceedings. J Clin Oncol 2004, 22 (abstract 679) 14S. 

Xufre, A., Albergaria, H., Inacio, J., Spencer-Martins, L, Girio, F. (2006). Application of fluorescence in situ hybridisation (FISH) to the analysis of yeast population dynamics in winery and laboratory grape must fermentations. International Journal of Food Microbiology, 108, 376-384. 

Tuohy KM, Finlay RK, Wynne AG, Gibson GR. A human volunteer study on the prebiotic effects of HP-Inulin — faecal bacteria enumerated using fluorescent in situ hybridisation (FISH). Anaerobe. 2001 7 113-118. 

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