Fibrinogen-coated surfaces

Peerscfake EIB. Glycoprotein lib and nia retention on fibrinogen-coated surfaces after lysis of adherent platelets. Blood 1995 82 3358-3363. 

The presence of low concentrations of proteins such as fibronectin and/ or von Willebrand factor can drastically alter the thrombotic response on a fibrinogen-coated surface, indicating that proteins that have yet to be characterized may contribute greatly to thrombosis. 

Types of antisera were added in different sequences following otherwise identical experiments, e.g. in Table VI, Exp. No. 1, anti-fibrinogen preceded anti-globulin serum, and in Exp. No. 2 the sequence was reversed. On the basis of previous calibrations with step-coated surfaces (110), the entries expressed in degrees can be multiplied by about 30 to obtain thickness in angstroms for a refractive index of about 1.60. 

Jen CJ, Lin IS. Direct observation of platelet adhesion onto fibrinogen-coated and fibrin- coated surfaces. Am JPhysiol 1991 261 H1457-H1463. 

Fibulin-1 is a glycoprotein that has been shown to be a component of the extracellular matrix that surrounds vascular smooth muscle. Fibulin-1 can bind to a number of matrix constituents including fibronectin, laminin, nidogen and fibrinogen. In fact, studies performed under flow conditions on fibulin-1 coated surfaces showed that this protein... 

The thrombotic response (the time vs. platelet and fibrin deposition pattern) for uncoated PVC (shown in Figures 2 and 3) is a response to PVC that is coated with a complex mixture of proteins in the initial seconds of blood contact. Therefore, at least part of the thrombotic response on PVC is generated by a complex protein-coated surface composed of many proteins adsorbed in various conformations, including serum albumin, 7-globulin, fibrinogen, and fibronectin. However, these four proteins account for only 75% of the total protein in plasma and, therefore, significant amounts of other proteins not accounted for by our measurements may be adsorbed to the test surface. 

Chick embryo muscle cell plating efficiency was low on albumin and y globulin coated surfaces but the fibrinogen and gelatin coated surfaces were strongly cell adherent. 

TABLE II. Overall Accounting for Platelets Arriving on Albumin-coated and Fibrinogen-coated Glass at 1.0 ml/min (80 s ) Basis 100 Platelets Contacting the Surface... 

Skarja, G. A., Brash, J. L., Bishop, R, and Woodhouse, K. A. (1998). Protein and platelet interactions with thermally denatured fibrinogen and crosslinked fibrin coated surfaces, BigmgiSJTols, 19,2129-2138. 

Jen CJ. Direct observation of platelet adhesion to fibrinogen- and fibrin-coated surfaces. Am J Physiol 1991 261 1457-63. 

Bovine serum albumin, fibrinogen and IgG (Serva, Heidelberg, FRG) were dissolved in PBS (pH = 7.2) at room temperature or, in the case of fibrinogen, at 37 C. Polymer discs were incubated in 1% solutions of the proteins for 1 or 24 hr. and washed afterwards with PBS. Contact angles of protein-coated surfaces were measured as described above. 

In other studies on the bacterial adhesion to protein-coated surfaces, a relationship between bacterial adhesion and the nature of the adsorbed protein was detected. If polyetherurethane samples were precoated with albumin or serum, a decrease in bacterial adhesion was always obtained for all strains tested compared to the uncoated polymer. However, precoating with fibrinogen led to an increase in adhesion for at least for one bacterial strain. Also, incubation of the polymer in 10% blood plasma for 1 hr. increased bacterial adhesion, while a 24 hr. plasma incubation did not have this effect. It is known that polyether-urethanes preferentially adsorb albumin from serum or plasma. In the case of blood plasma, we found that after 1 hr. incubation fibrinogen is the major protein adsorbed. However, this is replaced by albumin after a 24 hr. incubation period. We believe that the increase in adhesion is likely due to specific interactions between the fibrinogen layer and the particular bacterial strain. 

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