Fibrinogen-coated PVC

Figure 22. Fibrinogen-coated PVC after 15 min of blood contact (representing time of maximum deposition). Extensive fibrin network basal to attached platelets and platelet clumps is shown. Fibrin is the predominant species present.

Figure 19. Albumin-coated PVC after 5 min of blood contact. This blood contact time represents the maximum thrombus deposition as measured by radioisotope-labeled fibrinogen and platelets. No large adherent thrombi...

The thrombotic response (the time vs. platelet and fibrin deposition pattern) for uncoated PVC (shown in Figures 2 and 3) is a response to PVC that is coated with a complex mixture of proteins in the initial seconds of blood contact. Therefore, at least part of the thrombotic response on PVC is generated by a complex protein-coated surface composed of many proteins adsorbed in various conformations, including serum albumin, 7-globulin, fibrinogen, and fibronectin. However, these four proteins account for only 75% of the total protein in plasma and, therefore, significant amounts of other proteins not accounted for by our measurements may be adsorbed to the test surface. 

Fibrinogen and von Willebrand Factor. The thrombotic response on PVC coated with fibrinogen containing both fibronectin and von Willebrand factor, purified fibrinogen, and von Willebrand factor are discussed together because the responses appear to be interrelated. 

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