Extraction electrophoresis

Unlu, M., Morgan, M.E., Minden, J.S. (1997). Difference gel electrophoresis a single gel method for detecting changes in protein extracts. Electrophoresis 18, 2071-2077. 

Mateus, L., Cherkaoui, S., Christen, R, and Veuthey, J. L. (1999). Capillary electrophoresis-diode array detection-electrospray mass spectrometry for the analysis of selected tropane alkaloids in plant extracts. Electrophoresis 20, 3402—3409. 

Linden, M. V., Holopainen, J. M., Laukkanen, A., Riekkola, M. L., and Wiedmer, S. K., Cholesterol-rich membrane coatings for interaction studies in capillary electrophoresis application to red blood cell lipid extracts, Electrophoresis, 27, 3988-3998, 2006. 

M. Himmelsbach, W. Buchberger and C.W. Klampfl, Determination of antidepressants in surface and waste water samples by capillary electrophoresis with electrospray ionization mass spectrometric detection after preconcentration using off-hne sohd-phase extraction. Electrophoresis, 27,1220-1226, 2006. 

Posch TN, Martin N, Puetz M, Huhn C (2012) Nonaqueous capillary electrophoresis-mass spectrometry a versatile, straightforward tool for the analysis of alkaloids frran psychoactive plant extracts. Electrophoresis 33 (Copyright (C) 2012 American Chemical Society (ACS). All Rights Reserved.) 1557-1566. doi 10.1002/elps.201100682... 

Unger M, Laug S, Holzgrabe U (2005) Capillary zone electrophoresis as a tool for the quality control of goldenseal extracts. Electrophoresis 26 2430-2436. doi 10.1002/ elps.200410322... 

DETERMINATION OF POLYPHENOLIC ENANTIOMERS IN GREEN TEA EXTRACT BY CAPILLARY ZONE ELECTROPHORESIS... 

Catechin and epicatechin are two flavanols of the catechin family. They are enantiomers. The capillary zone electrophoresis (CE) methods with UV-detection were developed for quantitative determination of this flavanols in green tea extracts. For this purpose following conditions were varied mnning buffers, pH and concentration of chiral additive (P-cyclodextrin was chosen as a chiral selector). Borate buffers improve selectivity of separation because borate can make complexes with ortho-dihydroxy groups on the flavanoid nucleus. 

HCl aq EtOH). Recrystd from warm, acidified EtOH by addition of ammonia. The crude material (Ig) can be extracted with EtOH (50mL) in a Soxhlet apparatus for lOh to remove impurities. Impurities can be detected by paper electrophoresis. [Petrova et al. Anal Lett 5 695 1972.]... 

The natural world is one of eomplex mixtures petroleum may eontain 10 -10 eomponents, while it has been estimated that there are at least 150 000 different proteins in the human body. The separation methods necessary to cope with complexity of this kind are based on chromatography and electrophoresis, and it could be said that separation has been the science of the 20th century (1, 2). Indeed, separation science spans the century almost exactly. In the early 1900s, organic and natural product chemistry was dominated by synthesis and by structure determination by degradation, chemical reactions and elemental analysis distillation, liquid extraction, and especially crystallization were the separation methods available to organic chemists. 

Figure 11.19 SPME-CE analysis of urine samples (a) blank urine (a) directly injected and extracted for (b) 5 (c) 10 and (d) 30 min (b) Urine spiked with barbiturates, extracted for (e) 30 and (f, g) 5 min. Peak identification is as follows 1, pentobaitibal 2, butabarbital 3, secobarbital 4, amobarbital 5, aprobarbital 6, mephobarbital 7, butalbital 8, thiopental. Concenti ations used are 0.15-1.0 ppm (e, f) and 0.05-0.3 ppm (g). Reprinted from Analytical Chemistry, 69, S. Li and S. G. Weber, Determination of barbiturates by solid-phase microexti action and capillary electrophoresis, pp. 1217-1222, copyright 1997, with permission from the American Chemical Society.

D. Eigeys, A. Dua et and R. Aebersold, Identification of proteins by capillary electrophoresis-tandem mass specti ometiy . Evaluation of an on-line solid-phase extraction device , J. Chromatogr. A 763 295-306 (1997). 

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