Expression analysis/profiling research

Microfluidic devices that will enable global proteomic profiling will be particularly useful for biomarker discovery applications where the researcher is interested in comprehensively mapping all peptide/protein components in given biological fluids or tissues. Differential protein expression analysis will enable the discovery of specific protein markers or protein co-expression patterns that... 

Lin, W.H., Rui, Y.E., Hui, M.A., Xu, Z.H., Xui, H.W., 2004, DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments. Cell Research 14 34-45. 

A major obstacle to specific and sensitive detection of mature miRNAs in animals is their small size ( 19-23 nucleotides). Endlabeled LNA-probes, in which every third position is an LNA residue, have been used in Northern blot analysis to detect miRNAs with high specificity and showing at least 10-fold increased sensitivity compared with DNA probes (26), which is a major achievement in miRNA research. Furthermore, LNA probes with uniform Tm values of 72 °C have been used in high-sensitivity arrays for miRNA expression profiling (27). Such arrays allow discrimination between single nucleotide variations as demonstrated by the specific discovery of related miRNA family members. LNA probes have been used successfully to enhance the sensitivity and specificity of in situ hybridizations to miRNAs in zebrafish (28, 29) and in human brain (30). 

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