2- ethyl methane

Hsie, A.W., Brimer, P.A., Mitchell, T.J. and Gosslee, D.G. (1975). The dos-response relationship for ethyl methane sulfonate-induced mutation at the hypoxanthine-guanine phosphor-ibosyl transferase locus in Chinese hamster ovary cells. Somatic Cell Genet. 1 247-261. 

Ethyl methane-sulfonate Ethyl Selenac Ethyl TfeUurac Ethynodid diacetate ... 

Jacobs, M., Dolferus, R. Van Den Bossche (1988). Isolation and biochemical analysis of ethyl methane sulfonate induced alcohol dehydrogenase null mutants of Arabidopsis thaliana (L.) Heynth. Biochemical Genetics 26, 105-12. 

The test can be carried out on cultured cells or on cells from animals treated in vivo. In the former case the test chemical is usually evaluated in the presence and absence of the S-9 activation system from rat liver. Typically cells from a Chinese hamster ovary cell line are incubated in a liquid medium and exposed to several concentration of the test chemical, either with or without the S-9 fraction, for about 2 hours. Positive controls, such as ethyl methane sulfonate (a direct-acting compound) or dimethylni-trosamine (one that requires activation), as well as negative controls are also included. Test concentrations are based on cell toxicity levels determined by prior experiment and are selected in such a way that even at the highest dose excess growth does not occur. At the end of the treatment period the cells are washed, bromodeoxyuridine is added, and the cells are incubated for 24 hours or more. The cells are then fixed, stained with a fluorescent dye, and irradiated with UV light. Second division cells are scored under the microscope for SCEs (Figure 21.7). 

Neutral hydrolysis products of ethyl methane sulfonate. 

It is indispensable for any formal genetic analysis to be able to isolate mutants defective in genes coding for important cellular functions. In addition to the spontaneous mutations mentioned above, as caused by ISH elements, several protocols of induced mutagenesis were introduced. The most efficient method so far is the use of the alkylating agent ethyl methane sulfonate to cause point mutations... 

Conger, B.V., and J.V. Carabia. Mutagenic effectiveness and efficiency of sodium azide versus ethyl methane-sultonate in maize Induction of somatic mutations... 

Grow cells on enriched media containing the mutagen A-methyl-N, -nitro-Ar-nitrosoguanidine (MNNG) at 0.5/ig/ml or ethyl methane sulphonate (EMS) at 200/tg/ml for 16 h. 

EFC EGF EMS ESG ETC Eagle s MEM supplemented with foetal calf serum epidermal growth factor ethyl methane sulphonate Ewing sarcoma growth factor Eagle s MEM supplemented with tryptose phosphate (10%) and calf serum (10%)... 

SYNS EMS ENT 26,396 ETHYL ESTER of METHANESULFONIC ACID ETHYL ESTER of METHYLSL LFONIC ACID ETHYL ESTER of METHYLSULPHONICACID ETHYL METHANE-SULPHONATE ETHYL METHANSULFONATE ETHYL METHANSULPHONATE HALF-MYLERAN METHYNESULPHONIC ACID ETHYL ESTER METHYLSULFONIC ACID, ETHYL ESTER NSC-26805 RCRA I tYISTE NUMBER U119... 

The pentane which boils at 30° was formerly called di-methyl ethyl methane or simply iso-pentane, its structure being ... 

The hexane boiling at 64° was called methyl di-ethyl methane, as shown in the first formula. If, however, we write this structural formula differently, but representing the same structure, we see that the longest imbranched chain consists of five carbons. 

Pentanes Normal pentane Di-methyl ethyl methane (Iso-pentane)... 

N-(2-(4-Amino-N-ethyl-m-toluidino)ethyl)methane-sulfonamide sulfate (2 3). 

Corina and coworkers65 gave the El mass spectra of two fluorinated ethyl methane-thiosulfonates (95 and 96), which showed distinct molecular ions and preferential S—S bond cleavage, in analogy with unsubstituted 93a. However, the extent of fluorine substitution affected the site of charge retention, so that base peaks at m/z 78, [CH2FCHS]+, and m/z 79, [MeS02]+, were found for 95 and 96, respectively. 

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