Benchtop centrifuge

Benchtop centrifuge lEC Model HN-SII 6 x 50-mL fixed-angle solid rotor, 15-mL adapter sleeves Beckman pH meter... 

Problems that may arise with spectrophotometric assays may be due to turbid samples, inadequate mixing, or poor design of controls/blanks. Turbid samples should be clarified by centrifugation (a simple benchtop centrifuge at -5000 x g should be adequate) before reading the absorbance of the clear supernatant. 

Treat one aliquot at a time. Centrifuge in a benchtop centrifuge (Eppendorf centrifuge) for 2 s to spin down the cells. 

It is preferable to use a swing-out rotor to get a more compact pellet and minimize loss of yeast cells. We use a benchtop centrifuge with a swing-out rotor from Sorvall Instruments. 

This technique separates relatively heavy precipitates in low-speed benchtop centrifuges and is used most often for preparative scale separations. The technique is quite straightforward and consists in spinning the sample for a fixed period, the sample being contained in a tube or a bottle which has been inserted in the rotor of the centrifuge. After the spinning period is over, the sample tube is removed and the resulting two phases, pellet and supernatant (which should be readily apparent in the tube), may be separated by careful decantation. Further characterization or analysis is usually carried out on the individual phases. 

The preparation is finally purified with ion-exchange HPLC the sample is dissolved in 0.5—1 ml of 10 mM phosphate buffer, pH 6.0, and after removal of any insoluble material by centrifugation (Eppendorf benchtop centrifuge), up to 10 mg protein per run are loaded onto a 6 ml TSK 545 DEAE column (LKB, Sweden). The column is eluted at room temperature with a linear gradient of 0—0.3 M NaCl in the same phosphate buffer (flow rate 0.5 ml/min). Fractions of 0.5 ml are collected and analyzed for activator protein. 

The stems and roots of 10 plants from each of three containers from each group are prepared for 30% homogenate of plant material in buffer (0.01-M Tris-HCl pH 7.8, 7.5-mM PMSF, 2.5-mM EDTA, 325-pM bestatin, 3.5-pM E-64, 2.5-pM leu-peptin, and 0.75-pM aprotinin). The homogenate is centrifuged at 4°C and 12,000g in a benchtop centrifuge for 2h, and the supernatant is stored at -80°C for the GST and GPX assay. 

Refrigerated benchtop centrifuge, (CENTRA-7R, International Equipment Company, Needingham Heights, MA). 

Wash the giant liposomes by centrifugation over solution 5 in a benchtop centrifuge at 600 g for 5 min. Resuspend the liposomal pellet in 1 ml PBS. 

A refrigerated benchtop centrifuge equipped with a swing-out rotor capable of accommodating the same tubes as used for the high-speed spin, a cooled water bath at 10°C, and either a water bath or incubator at 21°C are required. 

Pellet the remaining cells at 3500 r.p.m. in a Sorvall RT7 benchtop centrifuge (PN11053 bucket with a 00438 insert), resuspend the cell pellet in approx. 0.5 ml of 2TY, and spread on one 243 x 243 mm 2TYGA plate. Incubate the plates at 30 C overnight... 

Centrifuge the overnight culture plate at 2000 r.p.m. for 10 min in a Sorvall RT7 benchtop centrifuge. Transfer 100 pi of each culture supernatant to the appropriate well of a microtitre plate containing 20 pi of 6 x PBS containing 18% (w/v) skimmed milk powder. Mix by pipetting and leave scFv to block for 1 h at room temperature. 

Tischzentrifuge tabletop centrifuge, benchtop centrifuge (multipmpose c.)... 

Kochsalz (NaCl) tablet density/pellet density Stopfdichte tabletop centrifuge/ benchtop centrifuge (multipurpose centrifuge) Tischzentrifuge tack vb heften, kleben,... 

Since degenerating antibodies tend to form larger complexes that stick nonspecifically to tissue and thus lead to increased background staining, we recommend centrifuging all antibody solutions for 10 min at max speed in a benchtop centrifuge. 

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