Epidermal growth factor fluorescent

Gadella, T. W. J. and Jovin, T. M. (1995). Oligomerization of epidermal growth-factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy—a stereochemical model for tyrosine kinase receptor activation. J. Cell Biol. 129, 1543-58. 

Another TIR/FRAP study on biological cell membranes has examined the reversible but specific binding kinetics of fluorescence-labeled epidermal growth factor to the surface of cells.(125) The background problem here was solved simply by choosing cells with a very large concentration of epidermal... 

E. H. Hellen and D. Axelrod, Kinetics of epidermal growth factor/receptor binding on cells measured by total internal reflection/fluorescence recovery after photobleaching, J. Fluor. 1, 113-128(1991). 

Lochner, N., Pittner, F., Wirth, M., Gabor, F., Wheat germ agglutinin binds to the epidermal growth factor-receptor of artificial Caco-2 membranes as detected by silver nanoparticle enhanced fluorescence. Pharm Res 20, 833-839 (2003). 

The use of recombinant inhibitors becomes easy when the protein is attached to green fluorescent protein (GFP), which eases the interpretation of the data [e.g., in the case of the Epsl5 mutant GFP-EA95/295 (epidermal growth factor receptor (EGFR) pathway substrate clone 15 mutant), a regulatory protein of clathrin assembly (57,58)]. 

Carter RE, Sorkin A. Endocytosis of functional epidermal growth factor receptor-green fluorescent protein chimera. J Biol Chem 1998 273(52) 35000-35007. 

Rosenthal EL, Kulbersh BD, King T, Chaudhuri TR, Zinn KR (2007) Use of fluorescent labeled anti-epidermal growth factor receptor antibody to image head and neck squamous cell carcinoma xenografts. Mol Cancer Ther 6(4) 1230-1238... 

Fig. 5.6 Fluorescence microscopy illustrating binding of fluorescein-conjugated human epidermal growth factor (hEGF) to the cell membrane of a single MDA-MB-458 human breast cancer cell, followed by internalization into cytoplasmic vesicles,...

Fluorescence resonance energy transfer (FRET) detects time and location of activation of Ras protein in live cells triggered by epidermal growth factor. [Michiyuki Matsuda, Research Institute for Microbial Diseases, Osaka University.]... 

Fuller and co-workers employed a substitution reaction in their synthesis of a fluorescent photo-affinity probe, AX7593, for the detection of epidermal growth factor receptor (EGFR) signal toansduction processes associated with solid tumor formation. Reaction of 7-(benzyloxy)-4-chloro-6-methoxyquinazoline and 1,3-diaminobenzene in die presence of hot isopropanol generated the desired 4-quinazoline in 97% yield. Five additional steps (not shown) were required to access AX7593. 

K. V El-Naggar, A. K. Gillenwater, A. M. Richards-Kortum, R. R. A far-red fluorescent contrast agent to image epidermal growth factor receptor expression. Photochem. Photobiol. 2004, 79, 272-279. 

Recently, Chariou and coworkers used filaments formed by the plant PVX to functionalize with a 12-amino acid peptide ligand GEll with affinity towards epidermal growth factor receptor. The fluorescently labeled PVX-GEl 1 enabled image and detection of different cancer cell lines (Chariou et al., 2015). 

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