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Enzyme sensors applications

Hoshi T, Anzai JI, Osa T (1994) Electrochemical deposition of avidin on the surface of a platinum electrode for enzyme sensor applications. Anal Chim Acta 289(3) 321-327... [Pg.567]

Anzai JI, Hoshi T, Osa T (1993) Electrochemical preparation of active avidin films for enzyme sensor applications. Chem Lett 7 1231-1234... [Pg.567]

Enzyme-based optical sensor applications will be further described in this book. They are still the most widespread optical biosensors but work is needed to overcome limitations such as shelf life, long term stability, in situ measurements, miniaturization, and the marketing of competitive devices. [Pg.350]

Diffusion Currents. Half-wave Potentials. Characteristics of the DME. Quantitative Analysis. Modes of Operation Used in Polarography. The Dissolved Oxygen Electrode and Biochemical Enzyme Sensors. Amperometric Titrations. Applications of Polarography and Amperometric Titrations. [Pg.8]

The type of enzyme sensor described above is highly selective and can be sensitive in operation. There are obvious applications for the determination of small amounts of oxidizable organic compounds. However, it is perhaps too early to give a realistic assessment of the overall importance of enzyme sensors to analytical chemistry. This is especially so because of parallel developments in other biochemical sensors which may be based upon a quite different physical principle. [Pg.257]

However, because of the mostly very slow electron transfer rate between the redox active protein and the anode, mediators have to be introduced to shuttle the electrons between the enzyme and the electrode effectively (indirect electrochemical procedure). As published in many papers, the direct electron transfer between the protein and an electrode can be accelerated by the application of promoters which are adsorbed at the electrode surface [27], However, this type of electrode modification, which is quite useful for analytical studies of the enzymes or for sensor applications is in most cases not stable and effective enough for long-term synthetic application. Therefore, soluble redox mediators such as ferrocene derivatives, quinoid compounds or other transition metal complexes are more appropriate for this purpose. [Pg.96]

This sensitivity to slow electron transfer kinetics could, however, prove to be an advantage in sensor applications where a mediator, with fast electron transfer kinetics, is used to shuttle electrons to a redox enzyme [82]. Chemical species that are electroactive in the same potential region as the mediator can act as interferants at such sensors. If such an interfering electroactive species shows slow electron transfer kinetics, it might be possible to eliminate this interference at the NEE. This is because at the NEE, the redox wave for the kinetically slow interferant might be unobservable in the region where the kinetically fast mediator is electroactive. We are currently exploring this possibility. [Pg.22]

Stabilization of activated oxidoreductases on time scales of months to years has historically been challenging, and the lack of success in this regard has limited the industrial implementation of redox enzymes to applications that do not require long lifetimes. However, as mentioned in the Introduction, some possibility of improved stability has arisen from immobilization of enzymes in hydrophilic cages formed by silica sol—gels and aerogels, primarily for sensor applications.The tradeoff of this approach is expected to be a lowering of current density because... [Pg.645]

Analytes are also used to specify the application. Glucose enzyme sensor is an enzyme biosensor measuring the glucose. Characteristics and commercial varieties of enzyme electrodes, especially using glucose oxidase, have been extensively reviewed by Kuan and Guilbault (17). [Pg.334]

Stability, duration, sensitivity, interference, and availability of substrates to contact enzymes are the criteria for the success of an enzyme sensor. These criteria depend on sources of enzymes, immobilization techniques, and transducers used. Food matrices are much more complicated than the clinical samples, hence, these criteria become extremely important for the application of the enzyme sensor in food analysis. An extensive list of the response time, detection limits, and stability of biosensors was summarized by Wagner (59). [Pg.337]

S. J. Setford, S.F. White and J.A. Bolbot, Measurement of protein using an electrochemical bi-enzyme sensor, Biosens. Bioelectron., 17 (2002) 79-86. P. Sarkar and A.P.F. Turner, Application of dual-step potential on single screen-printed modified carbon paste electrodes for detection of amino acids and proteins, Fresenius J. Anal. Chem., 364 (1999) 154-159. [Pg.549]

Aizawa presented an overview on the principles and applications of the electrochemical and optical biosensors [61]. The current development in the biocatalytic and bioaffinity bensensor and the applications of these sensors were given. The optical enzyme sensor for acetylcholine was based on use of an optical pH fiber with thin polyaniline film. [Pg.75]

A new development in the field of potentiometric enzyme sensors came in the 1980s from the work of Caras and Janata (72). They describe a penicillin-responsive device which consists of a pH-sensitive, ion-selective field effect transistor (ISFET) and an enzyme-immobilized ISFET (ENFET). Determining urea with ISFETs covered with immobilized urease is also possible (73). Current research is focused on the construction and characterization of ENFETs (27,73). Although ISFETs have several interesting features, the need to compensate for variations in the pH and buffering capacity of the sample is a serious hurdle for the rapid development of ENFETs. For detailed information on the principles and applications of ENFETs, the reader is referred to several recent reviews (27, 74) and Chapter 8. [Pg.78]

Diffusion currents. Half-wave potentials. Characteristics of the DME. Quantitative analysis. Modes of operation used in polarography. The dissolved oxygen electrode and biochemical enzyme sensors. Amperometric titrations. Applications of polarography and ampero-metric titrations. [Pg.531]

Less wide-spread than the enzymatic determination of these amino acids is the application of enzyme sensor systems for precursors or other amino acids. Collins et al. [116] described a flow injection system for the determination of a-ketoglutarate during an industrial fermentation. The system was based on glutamate dehydrogenase (Eq. (11.5)) and glutamate oxidase (Eq. (11.4))... [Pg.199]

Recently, Schuhmann et al. reported ethanol biosensors by entrapping quinohemoprotein alcohol dehydrogenase and Os-complex-modified poly(vinyl imidazole) during the electrochemically induced deposition of the poly(acrylate)-based resin [80]. The sensor exhibited its efficiency and also sufficient stability for practical applications. Author claims that the reported sensor preparation process is simple, easy to control, oxygen insensitive and can be applicable to other enzyme sensors. [Pg.349]

This chapter reviewed the development of polymeric electron transfer systems and discussed their applications to enzyme biosensors. Two major developments of polymeric electron transfer systems, poly[(VP)Os(bpy)2Cl " ] and its derivatives based hydrogels and mediator containing flexible polymeric electron transfer systems in a carbon paste and their applications to enzyme sensors were reviewed in terms of... [Pg.365]


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See also in sourсe #XX -- [ Pg.258 ]

See also in sourсe #XX -- [ Pg.258 ]




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