Enzyme and Immunoassays

P. A. Cunniff, ed.. Official Methods of Analysis of AO AC International, 16th ed., Vols. I and II, AO AC International, Arlington, Va., 1995. Vol. I includes Pesticide Formulations and Pesticide Residues. Over 2100 coUabotatively tested, approved methods for chemical and microbiological analyses, with 149 new methods, 103 revised/updated methods, methods using anibody-based test kits, enzyme immunoassay, and annual supplements containing new and revised methods chemical and common names of all dmgs and pesticides easy-to-locate references. 

Koup, J.R. and Brodsky, B., Comparison of homogeneous enzyme immunoassay and high pressure liquid chromatography for the determination of theophylline concentration in serum, Am. Rev. Respir. Dis., 117,1135,1978. 

M15. Marz, W., Siekmeier, R., Gross, E., and Gross, W., Determination of lipoprotein(a) Enzyme immunoassay and immunoradiometric assay compared. Clin. Chim. Acta 214, 153-163 (1993). 

E. Ishikawa, M. Imagawa, S. Hashida, S. Yoshitake, Y. Hamaguchi, andT. Ueno, Enzyme-labeling of antibodies and their fragments for enzyme immunoassay and immunohistochemical staining, J. Immunoassay 4, 209-327 (1983). 

Avrameas, S. (1983). Enzyme immunoassays and related techniques development and limitations. Curr Top Microbiol Immunol 104 93-9. 

The number of pollutants that are currently regulated constitute only a fraction of those found in the environment. In addition, their chemical characteristics and concentrations may vary widely. New and alternative methodologies that are simple, rapid, and reliable need to be developed. Enzyme immunoassay and portable GC and IR techniques need greater attention. 

Dankwardt, A., E.M. Thurman, and B. Hock (1997). Terbuthylazine and deterbuthylazine in rain and surface water Determination by enzyme immunoassay and gas chromatography/mass spectrometry. Ada. Hydrochim. Hydrobiol., 25 5-10. 

Del Valle, P.L. and J.O. Nelson (1994). Evaluation of atrazine soil extraction methods for the determination by enzyme immunoassay and gas chromatography. Arch. Environ. Contam. Toxicol., 27 375-383. 

Schewes, R., S. Wiist, J. Lepschy-von Gleissenthall, F.X. Maidl, A. Suss, B. Hock and G. Fischbeck (1994). Determination of weathered atrazine residues in soil by enzyme immunoassay and HPLC An evaluation study. Anal. Lett., 27(3) 487-494. 

Gruessner, B. and M.C. Watzin (1995). Patterns of herbicide contamination in selected Vermont streams detected by enzyme immunoassay and gas chromatography/mass spectrometry. Environ. Sci.Technol., 29 2806-2813. 

Castro et al made comparative determinations of phenytoin by various methods. Serum from patients being treated with phenytoin were analyzed for the drug by spectrophotometry, gas chromatography, radioimmunoassay, enzyme immunoassay and liquid chromatography. The assay values obtained were compared statistically. Enzyme immunoassay and liquid chromatography appear to be attractive alternatives to the more traditional methods of spectrophotometry and gas chromatography. 

Abad, A., M.J. Moreno, R. Pelegrf, et al. 1999. Determination of carbaryl, carbofuran and methiocarb in cucumbers and strawberries by monoclonal enzyme immunoassays and high-performance liquid chromatography with fluorescence detection An analytical comparison. J. Chromatogr. A 833 3-12. 

W10. Werle, E., Blazek, M., and Fiehn, W., The clinical significance of measuring different anti-dsDNA antibodies by using the Farr assay, an enzyme immunoassay and a Chrithidia luciliae immunofluorescence test. Lupus 1, 369—377 (1992). 

S. Y. Kim, Y-A. Jo, J. Choi, and M. J. Choi, Characterization of s-triazine antibodies and comparison of enzyme immunoassay and biotin-avidin enzyme immunoassay for the determination of s-triazine, Microchemical J. 68, 163-172 (2001). 

Maurer, H. H., Fritz, C. R, Toxicological Detection of Pholcodine and Its Metabolites in Urine and Hair Using Radio Immunoassay, Fluorescence Polarisation Immunoassay, Enzyme Immunoassay and Gas Chromatography-Mass Spectrometry, Int. J. Leg. Med., 104, 43,1990. 

H.H. Maurer and C.E Fritz. Toxicological detection of pholcodine and its metabolites in urine and hair using radio immunoassay, fluorescence polarisation immunoassay, enzyme immunoassay and gas chromatography-mass spectrometry. Int. ]. Leg. Med. 104, 43 6, 1990. 

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