Fluorescence polarisation immunoassay

In hair analysis, three methodically different lA with different kinds of labeling are used radioimmunoassay (RIA), which is the most common lA in hair analysis, enzymeimmunoassay (EIA), and fluorescence polarisation immunoassay (FPIA). In hair analysis, lA have the same advantages and disadvantages as compared to their use in urinalysis. They are fast, easy to handle, and can be automated. Their use can save time and expenses when a great number of negative samples has to be expected. 

Maurer, H. H., Fritz, C. R, Toxicological Detection of Pholcodine and Its Metabolites in Urine and Hair Using Radio Immunoassay, Fluorescence Polarisation Immunoassay, Enzyme Immunoassay and Gas Chromatography-Mass Spectrometry, Int. J. Leg. Med., 104, 43,1990. 

H.H. Maurer and C.E Fritz. Toxicological detection of pholcodine and its metabolites in urine and hair using radio immunoassay, fluorescence polarisation immunoassay, enzyme immunoassay and gas chromatography-mass spectrometry. Int. ]. Leg. Med. 104, 43 6, 1990. 

Fluorescence polarisation immunoassay, 154 Fluorescence spectrophotometers, 233 standardisation, 234... 

FPIA = Fluorescence Polarisation Immunoassay (Abbott, TD,). El A = Enzyme Immunoassay. 

A falsely elevated imipramine level was recorded when HPLC was used to determine serum imipramine levels in a patient taking imipramine, quetiapine, fluvoxamine, lithium and docusate. The abnormal readings were found to have been caused by a metabolite of quetiapine, and normal readings were obtained by altering the wavelength for detection of imipramine. Nortriptyline levels have been found to be falsely elevated in a patient also taking quetiapine when blood was analysed using fluorescence polarisation immunoassay, but were normal when an HPLC... 

Asian or Chinese ginseng (Panax ginseng) and Siberian ginseng (Eleu-therococcus senticosus) have both been found to interfere with some digoxin assays including fluorescence polarisation immunoassay (FPIA) and microparticle enzyme immunoassay (MEIA). ... 

Rhys Williams AT (1988) Fluorescence polarisation immunoassay. In Collins WP (ed.) Complementary Immunoassays, pp. 135-147. Chichester Wiley. 

The availability of MIP microparticles through this synthetic method has also stimulated the development of analytical techniques that make use of them as sensing elements. Apart from competitive radioassays [30] and immunoassays [32], which were already performed with ground bulk polymers, the small, regular size of the beads prepared by dispersion/precipitation polymerisation enables their use in CEC [45, 46], scintillation proximity assays [35], fluorescent polarisation assays [47], and chemiluminescence imaging [48]. 

There have been several new developments in fluorescence polarization immunoassay (FPIA). Lackowicz and Terpetschnig reviewed the use of long-lifetime metal-ligand complexes in fluorescence polarization assays [154, 155]. New complexes with Re(l) and Ru(II) were described for the highly sensitive detection of high-molecular weight analytes by FPIA. Laser-induced fluorescence polarisation detection has been used by Yatscoff and coworkers in capillary electrophoresis detection (CE-LIFP) [156]. For the analyte cyclosporin picomolar detection limits were attained. 

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