Enzymes evolution

Directed Enzyme Evolution Screening and Selection Methods, Humana Press, Totowa. Vol. 230. (b) Brakmann, S. and Johnsson, K. (eds)(2002) Directed Molecular Evolution of Proteins (or How to Improve Enzymes for Biocatalysis), Wdey-VCH Verlag GmbH, Weinheim. (c) Brakmann, S. and Schwienhorst, A. (eds)... 

Directed Enzyme Evolution and High-Throughput Screening... 

Kagami, O., Baik, S.-H. and Harayama, S. (2004) Effective DNA shuffling methods for enzyme evolution, in Enzyme Functionality (ed. A. Svendsen), Marcel Dekker, Inc., New York, pp. 425-441. 

Arnold, F.H. and Georgiou, G. (eds) (2003) Directed Enzyme Evolution Screening and Selection Methods, Methods in Molecular Biology, vol. 230, Humana Press, Inc., Clifton, NJ. 

Fox, R.J., Davis, S.C., Mundorff, E.C. et al. (2007) Improving catalytic function by ProSAR-driven enzyme evolution. Nature Biotechnology, 25, 338-344. 

Farinas, E.T., Bulter, T., Arnold, F.H., Directed enzyme evolution, Curr. Opin. Biotech-nol. 2001, 22, 545-551... 

Gupta, R.D. and Tawfik, D.S., Directed enzyme evolution via small and effective neutral drift libraries. Nat. Methods, 2008, 5, 939-942. 

Screening of Large Libraries and Directed Enzyme Evolution... 

Burbaum et al. considered how kinetic/thermodynamic features of present-day enzyme-catalyzed reactions suggest that enzyme evolution tends to maximize catalytic effectiveness. They analyzed Uni Uni enzymes in terms of reaction energetics. Catalytically optimized enzymes... 

Moore, J.C., Jin, H.M., Kuchner, O. and Arnold, F.H. (1997) Strategies for the in vitro evolution of protein function enzyme evolution by random recombination of improved... 

Senior, E., A. T. Bull, and J. H. Slater, Enzyme evolution in a microbial community growing on the herbicede Dalapon , Nature, 263, 476-479 (1976). 

Figure 12.5 Two cases of enzyme evolution. In both cases the enzymes bind the transition states equally well, but in (a) the substrate is bound strongly, and in (b) the enzyme has evolved to bind the substrate weakly ([S] is the same in both graphs). The activation energy in (a) is for ES —> ES, i.e., AG + AG, whereas in (b) it is for E + S — ES, i.e., AG. (The changes in Gibbs free energies are for the concentration of substrate used in the experiment, and not for standard states of 1 M.)...

Tao, H. and V.W. Cornish 2002. Milestones in directed enzyme evolution. Curr. Opin. Chem. Biol. 6 858-864. 

Directed enzyme evolution using the selection of phage-clisplayed enzymes is a powerful tool that represents an attractive alternative to the popular high-throughput screening technologies. 

G. Georgiou, Directed Enzyme Evolution (Screening and Selection Methods), Humana Press, Totowa, New Jersey, 2003. 

Csermely, P. (1997). Proteins, RNAs and chaperones in enzyme evolution a folding perspective. Trends in Biochem. Sci. 22,147-149. 

N. Cohen, S. Abramov, Y. Dror, and A. Freeman, In vitro enzyme evolution the screening challenge of isolating the one in a million, Trends Biotechnol. 2001, 19, 507-510. 

There are three dominant principles of enzyme evolution ... 

Fig. 1. A working strategy for directed enzyme evolution. The screening method should ensure that small enhancements brought about mainly by single mutations can be measured. The evolution of a new, useful enzyme requires an effective strategy for accumulating many such small improvements. Beneficial mutations can be accumulated in a sequential generations of random mutagenesis and screening or b by (random) recombination...

Love KR, Swoboda JG, Noren CJ et al (2006) Enabling glycosyltransferase evolution a facile substrate-attachment strategy for phage-display enzyme evolution. ChemBioChem 7 753-756... 

Graves, J.E. and Somero, G.N. (1982). Electrophoretic and functional enzymic evolution in four species of eastern Pacific barracudas from different thermal environments. Evolution 36,97-106. 

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