Enzyme Evolution to Enable Nonrecombinant Homoethanol Production

2 Enzyme Evolution to Enable Nonrecombinant Homoethanol Production 

As described above, the Z. mobilis homoethanol pathway was introduced to the E. coli genome because the native E. coli pathways expressed during fermentative 

This general strategy of using a feedback-resistant LPD mutant has been implemented by Genomatica to improve production of butanediol by . coli [131]. The specific mutation identified by Kim et al. was used to improve the production of -butanol, isobutanol, and pentanol by E. coli [132-134]. Thus, once again, metabolic engineering strategies that were first developed to improve ethanol production have been used to improve the production of other fuels and chemicals. 

---

S.2.4.2 Enzyme Evolution to Enable Nonrecombinant Homoethanol Production... 

---