Enzyme engineering, technology

Generation of Flavor and Aroma Components by Microbial Fermentation and Enzyme Engineering Technology... 

Since 1986 she has been engaged as a professor at the University of DUsseldorf and at the Research Centre Jtllich. She has received numerous honours (including Order of Merit of the Federal Republic of Germany in 1979, the Technology Transfer Prize of the German Research Minister in 1983, and the Enzyme Engineering Award, USA, in 1995). 

To evolve useful enzymes, genetic engineering technology has been applied increasingly to improve stability of enzymes, enantioselectivity, extension of substrate specificity for kinetic resolution of racemic compounds. Novel enzymes created by this technique will be available in large quantities and varieties within a next few years. In the near future, a lot of useful enzymes will be on the market and expanding number of chemists can use enzymes more freely than present due to the improvement in the simplification of experimental procedures. 

Chen, K., and Arnold, F. H. (1991). Enzyme engineering for nonaqueous solvents random mutagenesis to enhance activity of subtilisin E in polar organic media. Bio/ Technology, 9, 1073—1077. 

Helle, S., Cameron, D., et al., Effect of inhibitory compounds found in biomass hydrolysates on growth and xylose fermentation by a genetically engineered strain of Saccharomyces cerevisiae. Enzyme Microbial Technology 2003, 33 (6), 786-792. 

ADDmoNAL References Baldwin, T. O., F. M. Raushel, and A. I. Scott, Chemical Aspects of Enzyme Technology—Fundamentals, Plenum Press, New York, 1990. Various eds.. Enzyme Engineering, vols. 2-5, Plenum Press, New York, 1974-1980. Wingard, L. B., I. V. Berezin, and A. A. Klyosov (eds.). Enzyme Engineering—Future Directions, Plenum Press, 1980. 

The use of a-transglucosidases in the large-scale manufacture of novel bioderivatives is stiU limited by several factors such as enzyme selectivity, stability, and, in some cases, efficiency. To overcome these Umitations and further enlarge the appUcatimis of these enzymes, the latest protein engineering technologies have been used to tailor biocatalysts with specific properties for novel oligosaccharide. 

C Jacobs-Yonng, JA Heitmann, RA Venditti. Conventional kraft pulping using enzyme pretreatment technology Role of chip thickness, specie and enzyme combinations. In BN Brogdon, PW Hart, JC Ransdell, BL Scheller, eds. Innovative Advances in the Forest Products Industries. New York Amer Inst Chemical Engineers, 1998, pp. 1-15. 

The term enzyme engineering [11] (see Fig. 1) describes a range of techniques from deliberate chemical modification to remodeling a wild-type enzyme by gene technology. Subti-ligase , a mutant of subtilisin BNP , was prepared by Jackson et al. [12] by protein design and used in a further total synthesis of RNase... 

---