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EnVision staining system

Target Retrieval Solutions from DAKO with pH 6.0 or pH 9.0 are well-suited for use on formalin-fixed, paraffin-embedded tissue sections mounted on glass slides. Compared with 0.01 M Citrate buffer, pH 6, the use of 0.001 M EDTA buffer, pH 9, significantly improves staining results for many antigens, preserves the morphology better and is especially useful in combination with the Dako EnVision visualization systems. [Pg.49]

Rapid immunohistochemical study of frozen sections is necessary for intraoperative diagnosis in some cases. Rapid immunostaining is also helpful in confirming or excluding tumor clearance in resection margins or in detecting micrometastases in sentinel lymph nodes in breast cancer patients. Two methods to immunostain frozen sections are the enhanced polymer one-step staining (EPOS) system and the EnVision system both systems are detailed later. [Pg.138]

In contrast to the EPOS system, a modification of the highly sensitive two-step immunohistochemical EnVision system allows the detection of a broad spectrum of antigens in frozen sections in less than 13 min (Kammerer et al., 2001). In this study 38 out of 45 antibodies tested showed specific staining. In fact, the modified EnVision procedure allows the use of any suitable primary antibody, preferably monoclonal antibodies. Like the EPOS system, EnVision employs a dextran polymer coupled to horseradish peroxidase molecules for detection. No attempt was made to block endogenous peroxidase, nor was any antigen retrieval pretreatment used. Because of the very short incubation durations, a humid chamber is not required to avoid evaporation of immunoreagents. [Pg.139]

Figure 8. Transplanted kidney stained for C4d using (a) an ultrasensitive polymer detection system (EnVision) and (b) standard strepavidin-biotin peroxidase system. There was clear staining of peritubular capillaries in (b) compared to the polymer system where the stain extended into the intertubular interstitium masking the capillaries. Figure 8. Transplanted kidney stained for C4d using (a) an ultrasensitive polymer detection system (EnVision) and (b) standard strepavidin-biotin peroxidase system. There was clear staining of peritubular capillaries in (b) compared to the polymer system where the stain extended into the intertubular interstitium masking the capillaries.
Finally, it is important to be aware of the fact that visualization systems with dual recognition such as the EnVision + Dual Link System do not discriminate between species, and thus are only suitable for multiple staining when using the sequential method. Visualization kits with amplification layers that are not well specified should be avoided since possible cross-reactivity cannot be predicted. [Pg.106]

HIER treatment efficiently exposes endogenous biotin. This effect can lead to high background staining in tissues rich in mitochondria (e.g. the epithelia of kidney, liver, and gastrointestinal tissues). For this reason, we recommend the use of one of the newer nonbiotin-based polymer conjugate detection systems such as EnVision+ (Dako). [Pg.116]


See other pages where EnVision staining system is mentioned: [Pg.17]    [Pg.53]    [Pg.58]    [Pg.95]    [Pg.98]    [Pg.105]    [Pg.10]    [Pg.117]    [Pg.17]   
See also in sourсe #XX -- [ Pg.99 , Pg.138 , Pg.139 ]




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