Detectability of conjugates

Fluorescence of the diaminopyridine group allows detection of conjugates down to the pico-mole range, with excitation and emission maxima at 345 and 400 nm, respectively. For detection of BAP and its conjugates, the optimal buffer environment is less than pH 5, because its fluorescent properties are pH dependent. A preferred buffer is sodium acetate at pH 4. 

Corongiu, F.P. and Banni, S. 1994. Detection of conjugated dienes by second derivative ultraviolet spectrophotometry. Methods Enzymol. 233 303-310. 

Detection of conjugated dienes by high-performance liquid chromatography... 

Stone N, Faulds K, Graham D, Matousek P (2010) Deep-SERRS demonstration of deep Raman spectroscopy for non-invasive detection of conjugated SERRS nanoparticles buried within 25 mm of mammalian tissue. Anal Chem 82 3969-3973... 

Ultraviolet detection of conjugated dienes is simple, fast, and requires no chemical reagents and only small amounts of samples are needed. However, this... 

Banni, S., Day, B.W., Evans, R.W., Corongiu, E.R, and Lombardi, B. (1995) Detection of Conjugated Diene Isomers of Linoleic Acid in Liver Lipids of Rats Eed a Choline-Devoid Diet Indicates that the Diet Does Not Cause Lipoperoxidation, J. Nutr. Biochem. 6,281-289. 

Banni, S., A. Petroni, M. Blasevich, G. Carta, E. Angioni, E. Murra, B.W. Day, M.P. Melis, S. Spara and C. Ip. Detection of Conjugated C16 PUFAs in Rat Tissues as Possible Partial Beta-Oxidation Products of Naturally Occurring Conjugated Linoleic Acid and its Metabolites. Biochim. Biophys. Acta 1682 120— 27 (2004). 

Banni et al. (49) recently described the detection of conjugated C16 PUFA in rat tissues as possible partial peroxisomal P-oxidation products of naturally occurring CLA. As the fraction designated here as the mitochondrial fraction also contains a great part of the peroxisomes (50), the lower amount of CLA in the mitochondrial fraction of TC leads to the speculation that peroxisomes in TC show a stronger partial P-oxidation activity of CLA in comparison to healthy testicular tissue. The physiological properties of conjugated Cl6 PUFA need to be evaluated. 

More sensitive, nondestructive methods for determination of selected sugars after/separation are biological tests such as enzymatic reactions (82), and imunochemical detection (83). Imunostaining is particularly popular in molecular biochemistry and clinical chemistry research, due to the possibility of a direct detection of conjugated carbohydrates such as glycolipids (84). Detection of the... 

Addition of quercetin to culture cell medium enhanced the rate of growth of baby hamster kidney (BHK-21) cells and also diminished levels of Upid peroxidation breakdown products [214]. Furtoermore, quercetin and three other flavonoids, hesperetin, naringenin, rutin, were found to be effective inhibitors of lipid oxidation in two different in vitro experimental models (1) Fe( )-induced linoleate peroxidation, by detection of conjugated dienes, and (2) auto-oxidation... 

Eairs, N.J., Evershed, R.P., Quinlan, P.T., and Goad, LJ. (1989) Detection of conjugated and unconjugated steroids in ihe ovary eggs and hemolymph of the decapod crustacean Nephrops norve cus. Gen. Comp. Endocrinol., 74,199-208. 

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