Electrospray ionization oligosaccharides

Zamfir A, Seidler DC, Kresse H, Peter-Katalinic J. 2002. Structural characterization of chondroitin/dermatin sulfate oligosaccharides from bovine aorta by capillary electrophoresis and electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Rapid Commun Mass Spectrom 16 2015. 

Bahr, U., and Karas, M. (1999). Differentiation of isobaric peptides and human milk oligosaccharides by exact mass measurements using electrospray ionization orthogonal time-of-flight analysis. Rapid Commun. Mass Spectrom. 13 1052-1058. 

K. A. Thomsson, N. G. Karlsson, and G. C. Hansson, Sequencing of sulfated oligosaccharides from mucins by liquid chromatography and electrospray ionization tandem mass spectrometry,... 

Solouki, T., Reinhold, B.B., Costello, C.E. et al. (1998) Electrospray ionization and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance mass spectrometry of permethylated oligosaccharides. Anal. Chem., 70 (5), 857-64. 

Y. Fukuyama, M. Ciancia, H. Nonami, A. S. Cerezo, R. Erra-Balsells, and M. C. Matulewicz, Matrix-assisted ultraviolet laser-desorption ionization and electrospray-ionization time-of-flight mass spectrometry of sulfated neocarrabiose oligosaccharides, Carbohydr. Res., 337 (2002) 1553-1562. 

Several endeavors are being directed towards identifying biomarkers that can serve as a surrogate indicator of disease severity, in terms of either overall disease burden or involvement of a particular organ/system in patients with an LSD. In mucopolysaccharidosis type I, the analysis of the levels of oligosaccharides derived from GAGs in cultured fibroblasts (as measured by electrospray ionization tandem mass spectrometry) combined with the residual a-L-iduronidase activity have been shown to distinguish patients with and without CNS involvement (Fuller et al.,... 

LC ESI (8) mass spectra were recorded on a Finnigan TSQ700 instrument (San Jose, CA) in positive ion mode. A sample aliquot was injected into a fused silica microcapillary column with an inside diameter of 100 pm. The microcapillary was filled at the end with 10 cm of a C-18 reversed phase resin. PMP labeled oligosaccharides were eluted at ca. 1 pL/min directly into the electrospray ionization source with a 10 min gradient of acetic acid in H2O (0.5 %, v/v) to 80 % acetonitrile. Determination of experimental molecular weights was done with the deconvolution software provided by the manufacturer. 

J.-B. Lee, T. Hayashi, K. Hayashi, and U. Sankawa, Structural analysis of calcium spirulan (Ca-SP)-derived oligosaccharides using electrospray ionization mass spectrometry, J. Nat. Prod., 63(2000)136-138. 

Shen X, Perreault H (1999) Electrospray Ionization Mass Spectrometry of 1-Phenyl-3-methyl-5-pyrazolone Derivatives of Neutral and N-Acetylated Oligosaccharides. J Mass Spectrom 34 502... 

Brobera S, Nord LI, Kenne L (2004) Oligosaccharide Sequences in Quillaja Saponins by Electrospray Ionization Ion Trap Multi-Stage Mass Spectrometry. J Mass Spec-trom 39 691... 

The ionization source is the mechanical device that allows ionization to occur. Matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) are now the most common ionization sources for biomolecular MS, because both of them are soft ionization techniques that is, the sample ionization process generates few or no fragments even for large biomolecules, such as proteins and oligosaccharides, so that the intact molecular ions can be easily observed. 

Wolff, M. W., Bazin, H. G and Lindhardt, R. J. Analysis of fluorescently labeled oligosaccharides by capillary electrophoresis and electrospray ionization mass spectrometry. Biotechnol Tech, 13, 797, 1999. 

W. Mo, H. Sakamoto, A. Nishikawa, N. Kagi, J. I. Langridge, Y. Shimonishi, and T. Takao, Structural characterization of chemically derivatized oligosaccharides by nanoflow electrospray ionization mass spectrometry. Anal. Chem. 71, 4100-4106... 

A. S. Weiskopf, P. Vouros, and D. J. Harvey, Electrospray ionization-ion trap mass spectrometry for structural analysis of complex N-linked glycoprotein oligosaccharides, Anal. Chem. 70, 4441-4447 (1998). 

W. Chai, V. Piskarev, and A. M. Lawson, Branching pattern and sequence analysis of underivatized oligosaccharides by combined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray ionization mass spectrometry, J. Am. Soc. 

D. T. Li and G. R. Her, Strncmral analysis of chromophore-labeled disaccharides and oligosaccharides by electrospray ionization mass spectrometry and high-performance liquid chromatography/electrospray ionization mass spectrometry, J. Mass Spectrom. 33, 644-652 (1998). 

Dennhart, N., T. Fukamizo, R. Brzezinski, M.-E. Lacombe-Harvey, and T. Letzel. 2008. Oligosaccharide hydrolysis by chitosanase enzymes monitored by real-time electrospray ionization-mass spectrometry. J. Biotechnol. 134 253-260. 

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