Electrophoretic coatings phosphating

Chem. Descrip. Trifunctional acid ester with 161 ppm MEHQ inhibitor Uses Adhesion promoter in glass and metal coatings incl. tin-free steel, tinned steel, zinc phosphated steel, galvanized steel, electrophoretically coated steel, brass, aluminum, ironed aluminum used in beverage cans, laminating adhesives for metalized films Features Offers weatherability, chem. resist., flexibility, hardness, abrasion and water resist. 

Zawadzak et al. have considered the development of new tissue engineering scaffolds based on highly porous and interconnected PU foams electrophoretically coated with carbon nanotubes (Zawadzak et al., 2009). These authors observed that the presence of a uniform CNT coating on the surface of PU foams accelerated the formation of calcium phosphate, an indication of the material effectiveness to form strong bonds to bone tissue, when compared to the uncoated foams, which was related to favored calcium phosphate crystal nucleation and growth induced... 

FV 1 Capillary electrophoretic separation of five basic model nroteins in an epoxy-coated capillary. Experimental condition 65 cm separation length, inner diameter/outer diameter 50/363 m 0 OIM phosphate buffer, pH 7, 300 V/cm, 17 fiA. Peaks ... 

Ducheyne, P., Radin, S., Heughebaert, M., and Heughebaert, J.C. (1990) Calcium phosphate coatings on metallic porous surfaces. The effect of structure and composition on the electrophoretic deposition, vacuum sintering, and in vitro dissolution behavior. Biomaterials, 11, 244- 254. 

Figure 4 Simultaneous chiral separation of the isomeric tripeptides Gly-a-Asp-PheNH2 and Gly- -Asp-PheNHa. Experimental conditions 40/47cm polyacrylamide-coated capillary, 50 im, 50 mmol I sodium phosphate buffer, pH 5.25, 60mgmr car-boxymethyl- -cyclodextrin, -20 kV, UV detection at 215 nm. (Reprinted with permission from Sabah S and Scriba GKE (1998) Electrophoretic stereoisomer separation of aspartyl dipeptides and tripeptides in untreated fused-siiica and polyacrylamide-coated capillaries using charged cyclodextrins. Journal of Chromatography A 822 137-145 Elsevier.)...

Fig. 5A-C. Effect of treatment with dimethyl sulfate on the formation of poly(ADPR) nonhistones in cultured rat hepatocytes. Hepato-cytes were obtained from thyroidectomized male Wistar rats (180 g) and seeded on fib-ronectin-coated petri dishes (5 X 10 cells/ 100 mm dish). They were kept at 37 C applying 10 ml per dish of hormone and serum free medium [9] except for the first 3 h when dexainethasone (10" M) and insulin (10 M) were present. After 44 h in culture 40 tx of 0.1 M DMS (dissolved in DMSO) were added and the cells incubated for another 20 min (B). Control dishes were treated with DMSO alone (A) or with DMS plus 10 mM benzamide (C). Poly(ADPR) nonhistones were isolated as described in the legend of Fig. 1. Electrophoretic analysis was carried out in the presence of sodium dodecyl sulfate and urea using Tris/phosphate buffer at pH 6.0 [1]. Samples applied to the 7% gel corresponded to the content of 1 X 10" cells. Scans were performed from sihrer-stained gels (Bio-Rad kit) using a Hoefer densitometer (Hoefer Scientific, San Francisco)...

Another possibility is to use a capillary gel electrophoretic method that is nowadays a routinely and commercially available method for the determination of the molecular mass of proteins/polypeptides. This method can also be used for the separation of collagen chains and their polymers. For example, this procedure is described in the literamre for the separation of collagen type I a-chains and chain polymers p (dimers), and y (trimers), and also chain polymers of related molecular mass 300,(X)0 and higher (typically in the study of the formation of crosslinks). Besides commercially available kits, another option is to use fused-silica or poly vinylalcohol-coated capillaries filled with non-cross-linked polyacrylamide or hydroxyl-propylmethylcellulose in a 50 vaM Tris-glycine buffer (pH 8.8) or phosphate buffer (50 vaM, pH 2.5) (Table 1). 

The most important applications utilize a relatively thin coating of mixed iron and zinc phosphates on steel sheet as a standard pretreatment before painting of automotive bodies and other sheet-sted fabrications, e.g. refrigerators and washing machines. In the case of car bodies, electrophoretic priming (section 8.4) usually follows the phosphating stage. 

PUC 90] DUCHEYNE P., RADIN S., HEUGHEBAERT M. and HEUGHEBAERT J.C., Calcium phosphate ceramic coatings on porous titanium effect of structure and composition on electrophoretic deposition, vacuum sintering and in vitro dissolution . 

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