Electrophoresis of nucleic acids

Rill, RL Ramey, BA Van Winkle, DH Locke, BR, Capillary Gel Electrophoresis of Nucleic Acids in Pluronic F127 Copolymer Liquid Crystals, Chromatographia Supplement I, Vol 49, S65, 1999. 

Several research groups used another interesting column technology as an alternative to the modification of the capillary surface. This method is inherited from the field of electrophoresis of nucleic acids and involves capillaries filled with solutions of linear polymers. In contrast to the monolithic columns that will be discussed later in this review, the preparation of these pseudostationary phases need not be performed within the confines of the capillary. These materials, typically specifically designed copolymers [85-88] and modified den-drimers [89], exist as physically entangled polymer chains that effectively resemble highly swollen, chemically crosslinked gels. 

Mitchelson K.R., and Cheng J. (Eds), Capillary Electrophoresis of Nucleic Acids, Volume I Introduction to the Capillary Electrophoresis of Nucleic Acids Humana Press, Totowa. 

Rickwood D, Hames BD (eds.) (1984) Gel electrophoresis of nucleic acids a practical approach. IRL Press, Oxford Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual, 2nd ed., vol. 1, chap. 6, Cold Spring Harbor Laboratories Press Jany K-D, Hahn H (1991) In Bertram S, Gassen G (eds.) Gentechnische Methoden. pp. 39-43. Fischer, Stuttgart... 

Rickwood D, Homes BD (eds.) (1984) Gel electrophoresis of nucleic acids a practical approach. IRL Press, Oxford Voytas D (2000) Current protocols in molecular biology. 2.5A. WUey, New York... 

To understand how these modem methods work, it is necessary first to review some general laboratory techniques ubiquitous in genetic engineering. Among the most important are gel electrophoresis of nucleic acids, nucleic acid hybridization assays, and the polymerase chain reaction. 

B 6. Is it possible to use polyacrylamide as a matrix for electrophoresis of nucleic acids What are the limitations, if any ... 

Nucleic Acids Structure and Mapping Introduction Capillary Electrophoresis of Nucleic Acids Comparative Genomics Differential Display and Subtractive... 

Table 4-4 Recipes for Buffers Used in Agarose Cel Electrophoresis of Nucleic Acids and SDS-PAGE...

Rickwood, D., Hames, B.D. (1990) Gd Electrophoresis of Nucleic Acids 2nd Edition. IRL Press. 

One of the most felicitous features of electrophoresis of nucleic acids in gels is the dependence of the mobility on size. Consequently, if a calibration can be set up in terms of known species, gel electro-... 

Chapter 8. The practice of analytical gel electrophoresis of nucleic acids. . . 359 Chapter 9. Practice of preparative gel electrophoresis of nucleic acids. 408... 

Mitchelson, K. and J. Cheng (eds.), Capillary Electrophoresis of Nucleic Acids, Humana Press, To-towa, NJ, 2001. 

Ultrapure agarose for electrophoresis of nucleic acids (Life Technologies). 

D. Grierson, in Gel Electrophoresis of Nucleic Acids A Practical Approach, D. Rickwood and B. D. Hames, Eds., Oxford University Press, New York, 1982, pp. 29-31. 

Riesner D, Steger G, Zimmat R, Owens RA, Wagenhofer M, Hillen W, et al. Temperature-gradient gel electrophoresis of nucleic acids analysis of conformational transitions, sequence variations, and protein-nucleic, acid interactions. Electrophoresis 1989 10 377-89. 

Ferrance, J. P., Giordano, B. C., and Landers, J. P. Towards effective PCR-based amplification of DNA on microfabricated chips. In Capillary Electrophoresis of Nucleic Acids (K. R. Mitchelson and J. Cheng, Eds.). Humana Press, Clifton, NJ, 2001. 

Details of DNA electrophoresis methods are given in Table 9.3. The most widely used buffers in gel electrophoresis of nucleic acids are Tris/acetate/EDTA (TAE) and Tris/borate/EDTA (TBE). TBE has the best buffering capacity but the use of TAE tends to result in somewhat sharper bands. For some purposes, such as DNA extraction with glassmilk, TBE should be avoided unless sorbitol is... 

K. R. Mitchelson and J. Cheng Capillary Electrophoresis of Nucleic Acids-Introduction, Humana, 2004, ISBN 9781592590551. 

This chapter will describe the efforts taken to attempt to bring order to various electrophoretic methods by means of physical standards or standards activities. The discussion will not extend to electrophoresis of nucleic acids, which because of the nature of relative comparisons, needs to be addressed independently. 

Wenz, H. M., Dailey, D., and Johnson, M. D. (2001) Development of a high-throughput capillary electrophoresis protocol for DNA fragment analysis, in Capillary Electrophoresis of Nucleic Acids, Vol II. (Mitchelson, K. R., Cheng, J., eds.), Humana, Totowa, NJ, pp. 3-18. 

Capillary Electrophoresis of Nucleic Acids, Volume 2 Practical Applications of Capillary Electrophoresis, edited by Keith R. Mitchelson and Jing Cheng. 2001... 

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