Tris-borate-EDTA

Stellwagen, NC, Apparent Pore Size of Polyacrylamide Gels Comparison of Gels Cast and Run in Tris-acetate-EDTA and Tris-borate-EDTA Buffers, Electrophoresis 19, 1542, 1998. Stellwagen, NC Gelfi, C Righetti, PG, The Free Solution Mobility of DNA, Biopolymers 42, 687, 1997. 

Fig. 17.11. Bottom CGE separation of components of poly U (sigma) in 25% pluronic F127. Top Note the resolution of two contaminants between each of the oligonucleotides from about 15 to 27 nucleotides long in this expanded section of the bottom electropherogram. Electrophoresis was performed in 25% pluronic F127 in tris-borate-EDTA buffer (90 mM tris, 90 mM boric acid, 2 mM Na EDTA, pH 8.3.) (25°C, 500 V cm-1, effective column length 30 cm). Reprinted with permission from Ref. [82],...

Tris-borate-EDTA (TBE) buffer (lOx solution) 0.89 M TRIZMA base, 0.89 M H3BO3,20 mM EDTA. Per liter 108 g TRIZMA base, 55 g boric acid, 40 ml 0.5 M ethylenedi-aminetetraacetate (EDTA) solution. 

Standard research-grade agarose is usually sufficient, but special agaroses may be used for specific applications (e.g. high-resolution gels). The electrophoresis buffer is usually prepared and stored as a 10 x concentrated stock. The most commonly used buffer is Tris-borate-EDTA (TBE), or alternatively, one may use Tris-acetate-EDTA (TAE) buffer ... 

It is often convenient to make up buffer solutions at ten times normal strength (10 x) which can then be diluted accordingly. For example lOxTBE is ten times normal strength Tris-borate-EDTA buffer, x 1TBE is a ten-fold dilution with distilled water. 

To assay for the presence of supercoiled, circular mtDNA several approaches can be taken. The simplest method is to run a small part of the entire sample (1/20 of the total), either undigested or digested with a restriction enzyme, on a 0.8% (w/v) agarose gel in 1X TBE (Tris-borate-EDTA buffer) and to visualize the DNA by staining the gel with ethidium bromide. If the DNA is undigested by a... 

Aliquot 0.5-1 ml of the above solution into the pockets of a 0.45-0.55%, IX Tris-borate-EDTA (TBE) buffer18 (sterile), 2.7 pg/ml ethi-... 

Tris/Borate/EDTA (TBE) Buffer (0.5 x, working solution)... 

Figure 7.8 Schematic of the injection method for on-column hybridization. (j4) injection order and (B) after 200 V/cm field for 10 seconds. The high ionic strength the 5X Tris-Borate-EDTA (TBE) region causes focusing of the lower ionic strength target and probe, while HC1 removal induces renaturation. The probe in water mixes quickly into the target and anneals before the target renatures completely.

Fig. 2 Multiplex PCR profile of exons in Duchenne or Becker muscular dystrophy genes combined with two flanking standards. Capillary 40 cm (65 cm total length) X 75 tm polyacrylamide coated background electrolyte 0.5% poly(ethylene oxide), 1 MDa in IX Tris-borate-EDTA, lO tM aminoacridine, 2 nM Vistra Green field strength 108 V/cm detection LIF, 488 nm. [Reprinted with permission from/. Chromatogr. A 781 295 (1997), copyright 1997, Elsevier Science Publishers.]...

X Tris/borate/EDTA, pH 8.3 DNase-free, RNase-free, and protease-free buffer. 

Running Tris-Borate-EDTA (TBE) buffer (lx) 45 nM Tris-Borate, 1 mM Na EDTA pH 8.2. Store at room temperature. 

Details of DNA electrophoresis methods are given in Table 9.3. The most widely used buffers in gel electrophoresis of nucleic acids are Tris/acetate/EDTA (TAE) and Tris/borate/EDTA (TBE). TBE has the best buffering capacity but the use of TAE tends to result in somewhat sharper bands. For some purposes, such as DNA extraction with glassmilk, TBE should be avoided unless sorbitol is... 

Fig. 7. Image of the agarose gel to 2% in which the corresponding bands of control of bromophenol blue and xylenecyanol dyes (1) and DNA/monomaleimido-Nanogold 1.4 nm conjugate (l) are observed. Conditions 80V, electrophoresis time 20 min, using x0.5 Tris-borate-EDTA buffer as running buffer.

As a standard electrophoresis running buffer for polyacrylamide gels, 1.0-0.5% Tris-borate-EDTA (TBE) is used. To run electrophoresis, use low voltage ranging over 1-8 V/cm to prevent denaturation of small DNA fragments due to high temperature. 

Electrophoresis running buffer Tris-borate-EDTA, Tris-... 

Tris-borate-EDTA (TBE buffer) has more buffering capacity than TAE and provides a better resolution for the separation of small nucleic acid fragments (0.1-3.0kb). A 5x stock TBE solution has the following components ... 

Fig. 3 Appearance of fragments protected from micrococcal nuclease digestion following incubation of Xenopus sperm chromatin in homologous egg extract. Sperm nuclei were used without extract incubation (lane 2) or following incubation of 50 ng DNAZ/il egg LSS for 3 min (lane 3), 10 min (lane 4), 30 rain (lane 5), or 120 min (lane 6). Samples were diluted and the sperm nuclei were isolated by centrifugation. The samples were digested with micrococcal nuclease for 1 min and the DNA purified and separated by a 1.4% Tris-borate-EDTA agarose gel. Lane I shows molecular weight markers in lOO-base-pair increments.

TBC-NF. See Tri butyl citrate TBDMSIM. Seet-Butyidimethylsilyl imidazole TBDPE. See Tetrabromodipentaerythritol TBE TBE Buffer. See Tris-borate-EDTA TBEP TBEP. See Tributoxyethyl phosphate TBGE TBGE. See t-Butyl glycidyl ether... 

Tris-borate-EDTA agglutinant Triethyl citrate aggregate bonding modifier Alkyl (C12-14) glycidyl ether aggregation agent, polymer Dimethylethanolamine agric. 

Sodium tartrate Succinic acid Succinic anhydride Tetrasodium pyrophosphate Trisodium citrate buffer, gel electrophoresis Tris-borate-EDTA buffer, high pressure equip. 

Figure 7 Analysis of the 1-kb ladder by capillary zone electrophoresis. Conditions coated capillary of 100pm i.d., 47.4cm length, filled with 4.5% polyacrylamide strings in 100 mmol r Tris-borate-EDTA, pH 8.0. Sample a0.25pgmr solution of the 1-kb ladder was injected electrophoretically for 3 s at 4kV run at 4kV and 11.4pA. The numbers on each peak represent the length of each fragment, in number of bases. Tris, 2-amino-2-hydroxymethylpropane-1,3-diol EDTA, ethylenediaminetetraacetic acid.

High-viscosity gels (e.g., the high-Mw LPA) require either in situ polymerization or very high pressure to replace them in the capillary. In contrast, many of the low-viscosity polymer solutions do not require polymerization by the user. It is necessary only to dissolve a known amount of the polymer in basic buffers, such as tris-borate-EDTA (TBE) or 3-[[tris(hydroxymethyl)methyl]amino] propane-sulfonic acid (TAPS) (pH 8-9), or isoelectric buffers, such as His or Lys. Because the low conductivity of the isoelectric buffers minimizes Joule heating, high electric fields can be used for rapid separation. For oligonucleo-... 

Tris-borate-EDTA (TBE) buffer (5x) 445 mM Tris, 445 mM borate, 10 mM EDTA, prepared with RNase-ffee H2O. 

X TBE (Tris, borate, EDTA) electrophoresis buffer 445 mM Tris base (54.0 g). 445 mM boric acid (27.5 g), and 10 mM EDTA (3.72 g Na2EDTA2H20), adjust to 1 litre with distilled water... 

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