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Analysis of DNA fragments

Analysis of DNA Fragments after Digestion by Restriction Endonucleases... [Pg.124]

Agarose gel electrophoresis is an ideal technique for analysis of DNA fragments. In addition to the positive characteristics discussed, the technique is simple, rapid, and relatively inexpensive. Fragments that differ in molecular weight by as little as 1% can be resolved on agarose gels, and as little as 1 ng of DNA can be detected on a gel. Nucleic acids are visualized... [Pg.421]

Analysis of DNA fragments containing A, gene sequences from Origin ABC... [Pg.835]

Chen, Y.H., Chen, S.H., Analysis of DNA fragments by microchip electrophoresis fabricated on poly(methyl methacrylate) substrates using a wire-imprinting method. Electrophoresis 2000, 21, 165-170. [Pg.414]

HPLC, high performance (or pressure) liquid chromatography, is particularly suited for small water-soluble molecules and proteins. Most used for analysis of DNA fragments is the reverse phase HPLC. Detection with electrochemical detectors are preferred. There are many different brands of ECD detectors and electrodes/cells. In our laboratory we have found that the ESA Coulochem is working excellent for our purposes and provides excellent sensitivity. Similar experience with other detectors can be found. We have found that for HPLC-ECD analysis of urine, separation is critical due to electrochemically active peaks eluting close to that of 8-oxodG. Ways to detect a false peak is given in details elsewhere [3]. [Pg.34]

Ross, PL., PA. Davis, and P Belgrader. 1998. Analysis of DNA fragments from conventional and microfabricated PCR devices using delayed extraction MAL-DI-TOF mass spectrometry. Anal. Chem. 70 2067-2073. [Pg.113]

Figure 7 (A) Electron microscopic homoduplex analysis of DNA fragments containing... Figure 7 (A) Electron microscopic homoduplex analysis of DNA fragments containing...
Capillary electrophoresis (CE) is used to separate ionic species by exploiting their frictional forces and differences in the charges of the respective species. In traditional electrophoresis, electrically charged anal3des move in a conductive hq-uid medium under the influence of an electric field. CE has proved to be highly successful in enabling the analysis of DNA fragments and other biomolecules. Another commonly... [Pg.2017]

Intemucleosomal DNA fragmentation is the result of activation of the endogenous endonuclease in apoptotic cells. The traditional gel electrophoresis method for the analysis of DNA fragmentation is labor-intensive. However, a microfluidic device has been developed which integrates the selection of individual cells, the lysis of cells, and the separation of the released apoptotic DNA fragments. [Pg.2064]

PMMA) microchips. The matrices are suitable for the analysis of DNA fragments ranging from 20 bp to 40 kbp. [Pg.1611]

See other pages where Analysis of DNA fragments is mentioned: [Pg.806]    [Pg.436]    [Pg.436]    [Pg.35]    [Pg.206]    [Pg.78]    [Pg.36]    [Pg.270]    [Pg.162]    [Pg.634]    [Pg.442]    [Pg.42]    [Pg.805]    [Pg.3436]    [Pg.264]    [Pg.730]    [Pg.85]    [Pg.1214]    [Pg.144]   
See also in sourсe #XX -- [ Pg.124 , Pg.127 , Pg.416 , Pg.431 , Pg.432 , Pg.433 , Pg.434 , Pg.435 , Pg.436 , Pg.437 , Pg.438 , Pg.439 , Pg.440 , Pg.441 ]

See also in sourсe #XX -- [ Pg.124 , Pg.127 , Pg.416 , Pg.431 , Pg.432 , Pg.433 , Pg.434 , Pg.435 , Pg.436 , Pg.437 , Pg.438 , Pg.439 , Pg.440 , Pg.441 ]



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Analysis of DNA

DNA fragmentation

DNA fragments

Of DNA fragments

Sequence analysis of short DNA fragments

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