EDTA plasma

Specimen type Serum/EDTA plasma Serum/EDTA plasma... 

The bead-based technology works not only for inflammatory mediators that have diagnostic/prognostic valne, bnt also for others (e.g., C-reactive protein, IL-6). This can make bead-based systems even more powerful. Kofoed et al. combined in-house and commercially available kits and used bead-based Luminex systems to assay biomarkers of potential interest in EDTA-plasma samples (70). A 3-plex assay for suPAR, sTREM-1, MIF was added to a commercially available human cytokine panel, IL-1P, IL-6, IL-8, GM-CSF, and TNF-a. Compared to healthy controls, all eight analytes were significantly higher in plasma from bacterial sepsis patients. 

A mixture of EDTA-plasma samples of left-over blood of a large number of patients. Samples of 150 pi plasma are stored in Eppendorf vials at -20°C for a maximum of 1 year. Every series of analyses includes one pool plasma sample. Ten fatty acid results are plotted on an electronic Shewhart chart. 

Quantitative assay of biotinidase activity including evaluation for the presence of a Km defect requires 1 ml of plasma. Serum samples can be also used. Biotin therapy does not affect the assay [ 32 ]. The activity in plasma may decrease during storage (see Pitfalls and Limitations , below). We recommend the use of ethylene-diami-netetraacetic acid (EDTA)-plasma separated from whole blood and assayed within... 

EDTA-blood samples are collected after overnight fasting and cooled immediately on ice. EDTA plasmas are obtained by centrifugation at 4°C for 15 min at 800 xg, aliquoted, and can be stored at -70°C. 

Lankheet AG et al (2009) A validated assay for the quantitative analysis of vatalanib in human EDTA plasma by liquid chromatography coupled with electrospray ionization tandem mass spectrometry. J Chromatogr B Analyt Technol Biomed Life Sci 877 3625-3630... 

Fig. 6 Concentration-time profile of antidotal atropine and its enantiomers S- and / -hyoscyamine in plasma of an in vivo swine study. Swine were topically exposed to the nerve agent VR (302 pg/ kg, t0) followed by administration of atropine sulphate (30 pg/kg) and the reactivating oxime HI 6 (12.8 mg/kg) via three i.m. injections into the rear leg at 30 (I), 180 (II) and 330 min (III). Blood samples were collected at distinct time points to generate EDTA plasma. Maximum concentrations were found 4 min after drug administration each. No differences of S- and R-Hyo concentrations were evident underlining similar elimination kinetics for both enantiomers. Data are mean and SD from duplicate measurement using the enantioselective LC-MS/MS approach of John et al. [47,49]. Black circles, total hyo grey circles, S-hyo grey triangles, R-hyo...

If no changes during infusions but only postinfusion valves are monitored, C3d determined in EDTA-plasma is the best parameter to retrospectively confirm involvement of the complement system (Fig. 9). 

The presence of the different suPAR variants in EDTA plasma from a prostate cancer patient was analyzed by size exclusion chromatography and measurement of the fractions obtained with the three different TR-FIAs. This EDTA plasma contained detectable amounts of uPAR(I) in contrast to an EDTA plasma pool from healthy donors. The levels of suPAR measured with TR-FIA 2 were considerably higher and eluted at a later position in the patient sample than in the donor plasma, thus indicating the presence of suPAR(II nf) in the patient sample [108], In a recent study, the concentrations of uPAR(I) as well as the calculated suPAR(II-III) were found to be significantly elevated in serum samples from patients with prostate cancer compared to the concentrations in serum from men with benign prostatic conditions. Specific measurements of uPAR(I) were found to improve specificity of prostate cancer detection [142],... 

For calibration, a seven-point standard curve ranging from 2-32 ng/mL in rat or dog EDTA plasma was used. The four-parameter logistic model was used to describe the relationship between the OD readings and nominal concentration (CONC) of the analyte (DeLean et al. 1978) ... 

Futhan stabilized complement activation in EDTA plasma for extended periods of time at 4°C, as measured by RIA. Background levels of C3a and C4a in stabilized plasma from whole blood drawn into Futhan+EDTA and stored at 4°C for up to 48 hours showed only minimal ex vivo activation (Table I). EDTA effectively inhibited ex vivo generation of C3a, whereas there was an ongoing production of C4a. Heparin also appeared to keep the C3a, but not the C4a, at a low level. Heparin + EDTA was no better in preventing ex vivo C3a generation than EDTA or heparin alone, but yielded a marked improvement in the stabilization of C4a. Indeed, C4a levels remained almost as low with this combination as with Futhan. 

Whole blood was anticoagulated with lOmM EDTA, heparin (14U/ml), heparin + 5mM EDTA or 0.2mg/ml Futhan + lOmM EDTA. Plasma was separated from whole blood by centrifugation for 15 minutes at lOOOxg at 4°C. All samples were kept at 4° for the length of time indicated. Values are averages of two experiments except when marked with an asterisk for single values. 

Since we used active coagulation enzymes that are not necessarily generated in EDTA plasma, we can not rule out that continued classical pathway activation in EDTA plasma accounts for the ex vivo conversion of C4. 

Sample material Serum, heparin plasma, EDTA plasma or fluoride plasma. 

Sample material Acidified serum, acidified EDTA plasma or acidified heparin plasma. 

Sample material Serum, sodium heparinate plasma or EDTA plasma. 

Sample material Blood, EDTA blood, heparin blood, serum, EDTA plasma or heparin plasma. 

Principle The EDTA-plasma sample is applied onto the protective mesh. Integrated precipitation and lipoprotein fractionation by means of dextrane sulfate and magnesium ions then take place in the precipitation layer. The precipitated lipoprotein fractions - chylomicrones, VLDL (very low density lipoproteins) and LDL (low density lipoproteins) - are filtered by the transport layer and only the HDL (high-density lipoproteins) fraction reaches the indicator him where the HDL cholesterol concentration is determined enzymatically by the following reaction ... 

Influence of the volume of the sample (R24, R87). Determination of the glucose concentration depends on the dosage of the sample volume. If the volume is altered by 10%, an error of approx. 18 mg/dl or 1 mmol/1 may result. In the range between 30 to 32 1 (EDTA blood or EDTA plasma) there is no dependence on the volume (recovery 100 5%). New stripes showed no influence of the applied sample volume between 26 to 34 pi (RN6). 

EDTA blood (Reflotron) vs EDTA plasma (comparative method). 

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