Drug absorption studies

Ho NFH, JY Park, PF Ni, WI Higuchi. (1983). Advancing quantitative and mechanistic approaches in interfacing gastrointestinal drug absorption studies in animals and man. In WG Crouthamel, A Sarapu, eds. Animal Models for Oral Drug Delivery in Man In Situ and In Vivo Approaches. Washington, DC APh/APS, pp 27-106. 

Heylen, P., van den Mooter, G., Kinget, R., Drug absorption studies of prodrugs esters using the Caco-2 model evaluation of ester hydrolysis and transepithelial transport, Int. J. Pharm. 1998, 166, 45-53. 

Lu et al. (1992) performed a comparison of water and solute uptake in the in situ single-pass perfusion model and the isolated loops conscious rat model. Water flux in both experimental set-ups was found to be comparable. It was found that the solute (i.e. acetaminophen and phenytoin) membrane permeabilities (Pm) were consistently higher in the chronically isolated loops compared to the in situ perfusion. It was suggested that this was as a result of greater luminal fluid mixing in the in vivo system. A key advantage of the in vivo approach was that each animal can act as its own control for drug absorption studies. 

Chapter 6 The Isolated Perfused Lung for Drug Absorption Studies... 

Drug Absorption Studies Using the Isolated Perfused Lung... 

Chapter 9 In Vitro Cellular Models for Nasal Drug Absorption Studies 217... 

Ehrhardt C, Kneuer C, Fiegel J, Hanes J, Schaefer UF, Kim KJ, Lehr CM (2002) Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o- Implications for the use of this cell line as an in vitro model for bronchial drug absorption studies. Cell Tissue Res 308(3) 391-400. 

While a number of immortalised cell lines emanating from different cell types of the airway (i.e., tracheo-bronchial) epithelium of lungs from various mammalian species are available (see Chap. 10), reliable and continuously growing cell lines that possess AEC morphology and phenotype are not reported to date. Most studies use cell lines of alveolar epithelial origin for drug absorption studies, while the observations are hard or meaningless to extend to in vivo human situation. 

Steimer A, Franke H, Haltner-Ukomado E, Laue M, Ehrhardt C, Lehr CM (2007) Monolayers of porcine alveolar epithelial cells in primary culture as an in vitro model for drug absorption studies. Eur J Pharm Biopharm [Epub ahead of print] doi 10.1016/j.ejpb.2006.11.006... 

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