Isolated perfused lung

Keywords Isolated perfused lung Absorption Permeability Instillation Aerosolisation Drug disposition In vitro-in vivo correlation... 

Chapter 6 The Isolated Perfused Lung for Drug Absorption Studies... 

Ex Vivo Isolated Perfused Lung The use of the IPL allows lung-specific pharmacokinetics to be investigated without the contribution of systemic ADME. In the IPL, the structural and cellular integrity of the lung tissue, the permeability barriers and interaction between different cell types and biochemical activity... 

Figure 6.4 Experimental set-up for maintenance and monitoring of the isolated perfused lung [139].

Table 6.2 Physiological Parameters in Mouse, Rat, Rabbit and Human of Relevance to the Isolated Perfused Lung Preparation (Data from Davies and Morris, [24]). Respiratory Tidal Volume Can Be Calculated by Dividing Total Ventilation by Respiratory Rate.

Drug Absorption Studies Using the Isolated Perfused Lung... 

Figure 6.5 Relationship between drug absorption in vivo and airspace to perfusate transfer in the isolated perfused lung [142].

Bassett DJP, Roth RA (1992) The isolated perfused lung preparation. In Watson RR (ed.) In Vitro Methods of Toxicology. Boca Raton, CRC Press, Florida, pp 143-155. 

Kroll F, Karlsson JA, Nilsson E, Persson CG, Ryrfeldt A (1986) Lung mechanics of the guinea-pig isolated perfused lung. Acta Physiol Scand 128 1-8. 

Longmore WJ (1982) The isolated perfused lung as a model for studies of lung metabolism. In Farrel PM (ed.) Lung Development Biological and Clinical Perspectives. Academic Press Inc, New York, pp 101-110. 

Mehendale HM, Angevine LS, Ohmiya Y (1981) The isolated perfused lung-A critical evaluation. Toxicology 21 1-36. 

Niemeier RW, Bingham E (1972) An isolated perfused lung preparation for metabolic studies. Life Sci II 11 807-820. 

Sakagami M, Omidi Y, Campbell L, Kandalaft LE, Moris CJ, Barar J, Gumbleton M (2006) Expression and transport functionality of FcRn within rat alveolar epithelium a study in primary cell culture and in the isolated perfused lung. Pharm Res 23 270-279. 

Because of the paucity of appropriate AEC lines that form functional tight junctions, primary cultures of AEC are used for most in vitro studies of alveolar epithelial function (e.g., solute transport and metabolism). Primary mammalian AEC techniques involve isolation, purification and culture of ATII cells from tissues obtained either after lung resections or isolated perfused lungs. These... 

Niemeier RW (1984) The isolated perfused lung. Environ Health Perspect 56 35-41... 

Bingham, E., R. Niemeier, and W. Dalbey. Metabolism of environmental pollutants by the isolated perfused lung. Fed. Proc. 35 81-84, 1976. 

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