Drosophila isolation

SERCA-type -ATPases from non-mammalian cells (SERCAMED) Sequences of SERCA-type Ca -ATPases were also obtained from Plasmodium yoelii [68], Anemia [69] and Drosophila [70], These enzymes are similar in size to the SERCAl- and SERCA2a-type Ca -ATPases from mammalian muscles, but based on their N- and C-terminal sequences they represent a distinct group. In spite of the wide philogenetic variations between them they all share a common N-term-inal sequence (MED) that differs from mammalian enzymes. None of the corresponding proteins were isolated and characterized. 

Petruzzelli L, Herrera R, Arenas-Garcia R, Fernandez R, Bimbaum MJ, Rosen OM 1986 Isolation of a Drosophila genomic sequence homologous to the kinase domain of the human insulin receptor and detection of the phosphorylated Drosophila receptor with an anti-peptide antibody. Proc Natl Acad Sci USA 83 4710-4714 Podskalny J, McElduff A, Gorden P 1984 Insulin receptors on Chinese hamster ovary (CHO) cells altered insulin binding to glycosylation mutants. Biochem Biophys Res Commun 125 70-75... 

Gnagey, A.L., Forte, M. and Rosenberry, T.L. (1987) Isolation and characterization of acetylcholinesterase from Drosophila. Journal of Biological Chemistry 262, 13290-13298. 

Reproductive pheromones are powerful isolating agents between species, as is well known for insects such as moths and drosophilas (Wyatt, 2003). 

Figure 4.1 Separation of H3 9-17 trimethylated subsequentlydigested with trypsin. The resulting at l<9 from H3 9-17 monoacetylated. Histone peptides were analyzed by LC-MS/MSemploying H3 isolated from Drosophila melanogaster was LTQ-Orbitrap (Thermo Scientific) as detector, acylated with deuterated acetic anhydride and (a) Chromatogram of the analysis. The y axis...

The interaction between Drosophila, yeasts and columnar cacti of the Sonoran Desert has been the subject of much recent interest (37) As a coevolved system, perhaps more is known about this interaction than any other. The chemistry of the cacti (70 spp.) has been postulated to play a significant role in the establishment of this system (38 ), but this was based upon reports of a relatively small number of relatively simple alkaloids, and a small number of terpenoid compounds. Only recently, the diversification of plant compounds has been discovered to be much greater (5). In their section on alkaloids in the above work, BajaJ and McLaughlin report the presence of some thirty-five structures. In addition, I have been able to isolate some sixty triterpenoid glycosides (structures were not determined) and more were detected but not Isolated. 

A genetic screen for novel clock mutations in Drosophila sA to the discovery of the first kinase that plays a role in circadian rhythmicity (Price et al 1998). Doubletime dht) is a member of the casein kinase 1 (CKl) family, and is 86% identical to human casein kinase Is (CKls) within the kinase domain (Kloss et al 1998). Originally, two mutations were isolated that produce short (18 h dht ) and long (27 h dbt ) behavioural rhythms. Since then, many period-altering mutations and loss-of-function alleles have been described. The majority of these mutations reside in the kinase domain of dbt, altering its ability to bind and/or phosphorylate its substrate (Fig. 1). All of the DBT point mutations analysed alter the phosphorylation state and accumulation of PER, indicating that DBT affects... 

First isolated from human urine, biopterin (Fig. 15-17) is present in liver and other tissues where it functions in a reduced form as a hydroxylation coenzyme (see Chapter 18).338 It is also present in nitric oxide synthase (Chapter 18).341/342 Other functions in oxidative reactions, in regulation of electron transport, and in photosynthesis have been proposed.343 Neopterin, found in honeybee larvae, resembles biopterin but has a D-erythro configuration in the side chain. The red eye pigments of Drosophila, called drosopterins, are complex dimeric pterins containing fused 7-membered rings (Fig. 15-17).344 345... 

An experimental difficulty lies in the fact that there are only a few thousand taste buds in the tongue, with only 50-100 cells in a bud. They age rapidly, having a lifespan of only about ten days.924 There may be only 30,000-50,000 hard-to-isolate taste receptor cells on the tongue s surface.923 However, very recently published reports describe a large family of bitter and sweet receptors in mice and humans924-928 and in Drosophila.929 930 The sweet-sour receptors are thought to activate a G protein called gustducin,931/932 which plays a role similar to that of transducin in vision and... 

The biologic significance of Z DNA is currently unclear. However, several cellular proteins that bind specifically to Z DNA have been isolated from the nuclei of Drosophila fruit flies. The mere existence of such proteins suggests that they may function in some specific role when they encounter stretches of DNA that can adopt a Z conformation. 

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