DNA lesion recognition

Fromme JC, Verdine G.L., DNA lesion recognition by bacterial repair enzyme MutM, J. Biol. Chem., 2003, 278,51543-51548. 

A model for initial DNA lesion recognition by NER and MMR based on local conformational flexibility. DNA Repair, 4, 455-464. 

Fromme, J. C., and Verdine, G. L. (2003a). DNA lesion recognition by the bacterial repair enzyme MutM./. BioL Chem. 278, 51543—51548. 

RJ. Isaacs and H.P. Spielmann, A Model for Initial DNA Lesion Recognition by NER and MMR Based on Local Conformational Flexibility , DNA Repair, 2004,3,455... 

Compounds of this type are extremely important as they can be used for the preparation of catalytic antibodies, synthetic receptors, and model compounds for the investigation of the DNA lesion and recognition steps and DNA repair mechanisms (see Section 10.23.12). 

D. Mu et al., Recognition and repair of compound DNA lesions (base damage and mismatch) by human mismatch repair and excision repair systems. Mol. Cell. Biol. 17,760-769 (1997)... 

However, much data has been accumulated in recent years indicating that the replication machinery can elongate past cisplatin-DNA lesions in a mutagenic way [15], Intervention of specific DNA polymerases and protein-protein interactions between replicative enzymes and DNA damage-recognition proteins may lead to occasional translesion DNA synthesis. This translesion synthesis can occur in an error-prone fashion, leading to indue-... 

Cells deficient in DNA repair are hypersensitive to cisplatin and some cisplatin-resistant cell lines show increased DNA repair and increased expression of the DNA. Platinum-induced DNA lesions are primarily repaired by the NER system. This system involves more than 20 proteins responsible for damage recognition, incision of the DNA strand on both sides of the lesion, removal of the damaged bases, and finally ligation by a DNA ligase. 

Maillard, O., Camenisch, U., Blagoev, K.B., and Naegeli, H. (2008) Versatile protection from mutagenic DNA lesions conferred by bipartite recognition in nucleotide exdsion repair. Mutat. Res., 658, 271-286. 

DNA recognition mode in which the lesion is first recognized and verified in an ATP-dependent manner. Lesion removal then occurs by two latent endonucleases that are recruited to NER complexes in a subsequent step. In contrast to BER, damage recognition and incision activities do not reside in the same polypeptide, likely allowing NER to be a more adaptable system for lesion recognition and repair. 

Recognition and Removal of Bulky DNA Lesions by the Nucleotide Excision Repair System... 

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