Damage-recognition protein

DALI (software), 47 94 Damage recognition protein, HxlOP Dansylamide, binding to carbonic anhydrase, 22 379... 

However, much data has been accumulated in recent years indicating that the replication machinery can elongate past cisplatin-DNA lesions in a mutagenic way [15], Intervention of specific DNA polymerases and protein-protein interactions between replicative enzymes and DNA damage-recognition proteins may lead to occasional translesion DNA synthesis. This translesion synthesis can occur in an error-prone fashion, leading to indue-... 

The type of base modification will determine which protein will come first and which system, BER or NER, will be used to cure the damaged DNA. NER is responsible for the removal of bulky adducts such as dimerized pyrimidine bases produced by UV radiation from sunlight, or benzo(a)pyren or psoralen adducts and others (Tab. 1). Bulky adducts are Table 1. Radiation-induced DNA damage recognition proteins in human cells. 

Uvr A, a damage recognition protein, detects helical distortion caused by DNA adducts such as thymine dimers (a). It then associates with Uvr B to form the A2B complex. After binding to the damaged segments, A2B forces DNA to bend. Uvr A then dissociates (b). The binding of the nuclease Uvr C to Uvr B (c) and the action of the helicase Uvr D (d) results in the excision of a 12-nucleotide DNA strand (12-mer). After Uvr B is released (e) the excision gap is repaired by pol I (f). 

Lindsey-Boltz, L.A. and Sancar, A. (2007) RNA polymerase the most specific damage recognition protein in cellular responses to DNA damage Proc. Natl. Acad. Sci. USA, 104, 13213-13214. 

A similar approach was used to identify a mammalian cellular factor that selectively recognized adducts of active antitumor platinum compounds as well as individual adducts formed by cis-DDP on DNA (30). This factor was found in human HeLa and hamster V79 cells. The damage-recognition protein binds to cis-DDP platinated DNA at (D/N)t, 0.007... 

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