DNA isolation kit

Several standard DNA isolation kits are commercially available, including the QIAamp DNA Stool Mini Kit and the DNeasy Plant Mini Kit made by Qiagen. Both of these products are based on silica gel membrane technology and allow for the extraction of total DNA from processed foods and raw foodstuffs, respectively. In... 

QIAamp Blood Kit (QIAgen) total DNA isolation kit, based on protease... 

DNA isolation kits Wizard Plus Minipreps DNA Purification System (Promega, Madison, WI), Qiagen Plasmid Maxi kit (Qiagen, Chatsworth, CA). 

Perform genomic DNA isolation from S. aureus test and reference strains using a modification of the Edge Biosystems genomic DNA isolation kit. The modification consists of addition of lysostaphin (100 pg/mL final concentration) to bacterial cells and incubation at 37 °C for 10 min prior to DNA extraction. 

Slurry DNA isolation kits are available (e.g.. Bio 101 Geneclean Kits by Qbiogene,... 

PCR for DGGE Anafysis The first step in DGGE analysis is DNA extraction followed by PCR amplification of a target gene. There are a number of commercially available kits for DNA extraction from environmental samples. We routinely use the bead-beating-based PowerSoil DNA Isolation Kit (MO BIO Laboratories,... 

Here, we discuss an optimized DNA extraction method specifically for electrode-adhered biofilm communities. We describe the use of a biofilm DNA extraction kit from MoBio Laboratories Inc. to extract DNA from electrode-adhered communities (PowerBiofilm DNA Isolation Kit, www.mobio.com). Here, we included a slightly modified alternative for cell lysis to include less meehanical bead-beating and multiple heat treatments. This method for cell lysis has been found to be successful for electrogenic community analyses [18, 21], We do not endorse MoBio Laboratories. This protocol is solely meant to be a starting point for researchers. 

The MoBio PowerBiofilm DNA Isolation Kit includes detailed schematics and protocols for beginning and experienced users. The following is a summary of this protocol including some notes on the basis of our experience with DNA extraction from carbon cloth electrodes [23]. 

DNA was isolated from the gel block using the DNA extraction kit (Cytokine, Russia). DNA added to the tube containing the reagents for PCR (DNA-Technology, Russia) and amplified in 35 cycles (30s 93°C, 30s 59°C and 30s at 72°C). For subsequent analysis 5 pi of the product was analyzed by gel electrophoresis as described above. 

The assay of enzyme induction at the mRNA level is much easier to perform than the chemical assay of each individual enzyme and the response to a physiological event is intrinsically faster to detect. Moreover, mRNA is often more stable under cell extract preparation conditions than enzymes, if destruction by RNAses can be prevented. For analysing a number of different enzymes in parallel, only one sample preparation procedure is necessary, which produces a crude RNA extract, and only in the final step the different DNA or RNA probes are used for detection of specific enzyme RNAs. Recently, the detection of RNA is further facilitated by ready-to-use RNA isolation kits, non-radioactive DNA or RNA probes and the dot-blot and slot-blot techniques, respectively, [51,52]. 

JETSTAR Plasmid Kit (GENOMED) plasmid isolation kit, based alkaline lysis of the bacterial cell. Chromosomal DNA sticks to the cell wall and coprecipitates with the cell debris, whereas plasmid DNA remains solubilized. An anion exchange resin, provided in columns, binds the plasmid DNA under high salt conditions, the DNA is washed and eluted in low salt conditions. The solutions are provided in the kit El (cell resuspension buffer 50 mM Tris-HCl, pH 8.0, 10 mMEDTA), E2 (cell lysis solution 200 mM NaOH, 1% SDS), E3 (neutralization buffer 3.2 M KOAc/HOAc, pH 5.5), E4 (column equilibration... 

To determine optimum alcohol fixation conditions for the integrity and recovery of the RNA from the tissue, the extracts from each fixation were column-purified on RNA Arcturus Picopure RNA Isolation Kit , and residual DNA was degraded... 

To analyze your library for diversity, take 0.5 mL of dense yeast from the library and isolate plasmid from the yeast using the Zymoprep II kit. Use the DNA isolated from the yeast to transform into E. coli, culture at least 8-10 colonies, and isolate the plasmids from these cells. Sequence the plasmids using the YRS reverse primer and ensure that you have obtained a reasonable level of mutations through your error-prone library protocol. 

Isolate plasmid DNA from individual clones using commercially available yeast plasmid DNA miniprep kit (Zymoprep), according to manufacturer s instructions. Unlike typical pUC-based E. coll plasmids, only 1 copy of the CEN6/ARSH4 bi scd pYD 1 plasmid will be present per yeast cell. See Note 14 for alternative strategies to recover gene of interest for sequence analysis. 

Genomic DNA was isolated using the QIAamp DNA mini kit (Qiagen, Inc., Valencia, CA). 

Prepare pOK-target locus plasmid from E. coli using QIAprep Spin Kit (Qiagen), or another plasmid DNA isolation method. 

Plasmid DNA isolation reagents (Qiagen maxiprep and mini-prep kits). 

Perform multiplex PCRs following the manual for the Qiagen Multiplex PCR Kit. This often requires htde to no optimization. Use no template and yeast host DNA as negative controls. Use genomic DNA isolated from the bacterium of interest as a positive control. As another positive control, mix together yeast host DNA and genomic DNA isolated from the bacterium of interest. 

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