DNA diagnostics

Demidov VV New kids on the block emerging PNA-based DNA diagnostics. Expert. Rev. Mol. Diagn. 2002, 2 199-201. 

The development of DNA sensors and high-density DNA arrays has been prompted by the tremendous demands for innovative analytical tools capable of delivering the genetic information in a faster, simpler, and cheaper manner at the sample source, compared to traditional nucleic acid assays. Nanoparticle-biopolymer conjugates offer great potential for DNA diagnostics and can have a profound impact upon bioanalytical chemistry. Nanoparticle/polynucleotide assemblies for advanced electrical detection of DNA sequences have been reviewed by Wang [145]. 

Grozema FC, Siebbeles LDA, Gelinck GH, Warman JM (2005) The Opto-Electronic Properties of Isolated Phenylenevinylene Molecular Wires. 257 135-164 Guiseppi-Elie A, Lingerfelt L (2005) Impedimetric Detection of DNA Hybridization Towards Near-Patient DNA Diagnostics. 260 161-186 Guo Y, see Coleman AW (2007) 277 31-88... 

DNA CT also permits chemistry at a distance. Oxidative DNA damage and thymine dimer repair can proceed in a DNA-mediated reaction initiated from a remote site. These reactions too are sensitive to intervening DNA dynamical structure, and such structures can serve to modulate DNA CT chemistry. The sensitivity of DNA CT to base pair stacking also provides the basis for the design of new DNA diagnostics, tools to detect mutations in DNA and to probe protein-DNA interactions. 

D.C. Thomas, G.A. Nardone, and S.K. Randall, Amplification of padlock probes for DNA diagnostics by cascade rolling circle amplification or the polymerase chain reaction. Arch. Pathol. Lab. Med. 123, 1170-1176 (1999). 

Keywords DNA diagnostics Impedimetric detection DNA hybridization DNA immobilization... 

Sheldon, EL (1996) NIST-ATP Workshop on Tools for DNA Diagnostics, September 9, Gaithersburg, Maryland, USA... 

Towards Near-Patient DNA Diagnostics A. Guiseppi-Elie L. Iingerfelt... 

Applications of electrochemical transducers have relied on conventional and bulky disk (C, Au) or mercury drop electrodes, as well as on mass-producible, single-use, thick-film carbon screen-printed electrodes. The sensitivity of such devices, coupled to their compatibility with modern microfabrication technologies, portability, low cost (disposability), minimal power requirements, and independence of sample turbidity or optical pathway, make them excellent candidates for DNA diagnostics. In addition, electrochem-... 

Monaghan PB, McCamey KM, Ricketts A, Littleford RE, Docherty F, Smith WE, Graham D, Cooper JM (2007) Bead-based DNA diagnostic assay for chlamydia using nanoparticle-mediated surface-enhanced resonance Raman scattering detection within a lab-on-a-chip format Anal Chem 79 2844—2849... 

DNA diagnostics. Representations of sequencing gels for variants of the a chain of human hemoglobin are shown here. What is the nature of the amino acid change in each of the variants The first triplet encodes valine. 

Chen X, Livak KJ, Kwok PY. A homogeneous, hgase-mediated DNA diagnostic test. Genome Res 1998 8 549-56. 

Patrushev LI, Zykiva ES, Kayushin AL, Korosteleva MD, Miroshnikov Al, Bokarew IN, et al. New DNA diagnostic system for detection of factor V Leiden. Thrombosis Res 1998 92 251-9. 

Little, D. P., Cornish, T. J., O Donnell, M. J., Braun, A., Cotter, R. J., and Koster, H. MALDI on a chip analysis of arrays of low-femtomole to subfemtomole quantities of synthetic oligonucleotides and DNA diagnostic products dispensed by a piezoelectric pipet. Anal. Chem. 69 4540 546, 1997. 

H. MALDl on a Chip Analysis of Arrays of Low-Eemtomole to Subfemto-mole Quantities of Synthetic Oligonucleotides and DNA Diagnostic Products Dispensed by a Piezoelectric Pipet, Anal. Chem. 69,4540—4546 (1997). Nelson, R.W. Nedelkov, D. Tubbs, K.A. Biosensor Chip Mass Spectrometry A Chip-Based Proteomics Approach, Electrophoresis 21,1155-1163 (2000). 

N2. Nickerson, D. A., Kaiser, R., Lappin, S., Stewart, J., Hood, L., and Landegren, U., Automated DNA diagnostics using an ELISA-based oligonucleotide ligation assay. Proc. Nall. Acad. Sci. U.S.A. 87, 8923-8929 (1990). 

Drmanac, S., Kita, D., Labat, I., Hauser, B Schmidt, C Burczak, J. D., and Drmanac, R. (1998) Accurate sequencing by hybridization for DNA diagnostics and individual genomics. Nature Biotechn. 16, 54-58. 

Chemiluminescence (CL) methods assure good results for analysis of body fluids and tissues. This is demonstrated by urinary iodide,185 cholesterol,186 and serum glucose187 assay. The CL method has had a major impact on all forms of DNA diagnostic techniques.188 189 NMR spectroscopy can also be used for in vivo measurements for diagnosis of illness. For example, the relationships between fiber composition and NMR assessment in human... 

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