Impedimetric detection

Grozema FC, Siebbeles LDA, Gelinck GH, Warman JM (2005) The Opto-Electronic Properties of Isolated Phenylenevinylene Molecular Wires. 257 135-164 Guiseppi-Elie A, Lingerfelt L (2005) Impedimetric Detection of DNA Hybridization Towards Near-Patient DNA Diagnostics. 260 161-186 Guo Y, see Coleman AW (2007) 277 31-88... 

Keywords DNA diagnostics Impedimetric detection DNA hybridization DNA immobilization... 

F. Lucarelli, G. Marrazza and M. Mascini, Enzyme-based impedimetric detection of PCR products using oligonucleotide-modified screen-printed gold electrodes, Biosens. Bioelectron., 20 (2005) 2001-2009. 

Figure 4.2 Impedimetric detection of DNA oligonucleotides. Sensor preparation as in Figure 4.1. The accessibility of the gold surface for ferri-/ferrocyanide depended on the amount of negative charge accumulated on the electrode surface. DNA hybridization increased the negative charge density and yielded increased charge transfer resistance as measured by impedance spectroscopy.

P. Van Gerwen, K. Baert, and R. Rossau. Impedimetric detection system and method of production thereof. US Patent 6440662, assigned to Innogenetics N. V. (Ghent, BE), August 27,2002. 

The same group discussed recently described impedimetric detection methods for double-tagged DNA from PCR amplification of Salmonella spp. [77]. One of these methods involved amplification of the impedimetric signal by using a monoclonal IgGlkappa anti-... 

The most common immobilization of biomolecules onto an IME device for impedimetric detection is the covalent linkage by the use of organosUane chemistries on the exposed glass of the interdigit space and aUcanethiol chemistries on the gold digits. However, immobilization of the... 

In DNA immobilization, several factors, such as the line and space dimensions of the IME (cell constant), immobilization strategy, the nature of the immobilized DNA molecules, and the length of the DNA probe, may affect the final signal in impedimetric detection. For example, oligonucleotide probes that are oriented parallel to the electrode result in decreased conductance compared to oligonucleotides that are oriented perpendicular to the electrode. The increase in conductance is associated with increased current... 

Impedimetric Biosensors for Nano- and Microfluidics, Fig. 10 The principle of IME impedimetric detection of the antibody immobilization and the bacterial attachment, (a) Nyquist impedance spectra of the IME-based biosensor upon antibody immobilization and bacterial attachment with different number of E. coli 0157 H7 cells on their surface, a = antibodies only, b = 4.36 x 10 OTJ/ml, c = 4.36 x 10 CFUAnl, d = 4.36 x 10 CHJ/ml, and e = 4.36 x 10 CEU/ml. Data points from left to right correspond to decreasing frequency. Amplitude voltage 5 mV Electrolyte 10 mM [EefCNlg] (1 1) in 0.01 M PBS, pH 7.4. (b) The linear relationship betwetm the electron transfer resistance and logarithmic value of E. coli concentrations daived from Fig. 10a... 

The fabrication of DNA sensors was thus carried out by the electropolymerization of the chelating pyrrole-NTA ligand followed by the successive coordination of Cu " and ssDNA modified by a polyhistidine tag [16]. Then, the labeless impedimetric detection of a ssDNA from the human immunodeficiency virus was performed via its hybridization onto the polypyrrole surface. The resulting detection limit (10 mol L ) was markedly better than those currently recorded for impedimetric DNA sensors. 

Bongaers, E. Alenus, J. Horemans, F. Weustenraed, A. Lutsen, L. Vanderzande, D. Cleij, T.J. Troost, G.J. Brummer, R.-J. Wagner, P. (2010). A MIP-based biomimetic sensor for the impedimetric detection of histamine in different pH environments. Physica Status Solidi (A) Applications and Materials, 207, 837-843. 

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