Mass spectrometry on-chip

While an array of anal)ftical techniques has been developed to study various aspects of cells (please see cell assays in microfluidics), this chapter is focused on chemical cytometry. As mentioned above, chemical cytometry refers to methods in which intracellular constituents of a single cell are analyzed by means of a chemical separation. Such methods typically employ capillary electrophoresis for separations, combined with laser induced fluorescence (LIF) or amperometry for detection. We refer readers to other chapters for detailed descriptions of other methods used to evaluate contents of cells ( patch-clamp measurements on-chip, mass spectrometry on chip device). 

H. MALDl on a Chip Analysis of Arrays of Low-Eemtomole to Subfemto-mole Quantities of Synthetic Oligonucleotides and DNA Diagnostic Products Dispensed by a Piezoelectric Pipet, Anal. Chem. 69,4540—4546 (1997). Nelson, R.W. Nedelkov, D. Tubbs, K.A. Biosensor Chip Mass Spectrometry A Chip-Based Proteomics Approach, Electrophoresis 21,1155-1163 (2000). 

Liljegren, G., Dahlin, A., Zettersten, C., Bergquist, J., Nyholm, L., On-line coupling of a microelectrode array equipped poly(dimethylsiloxane) microchip with an integrated graphite electrospray emitter for electrospray ionization mass spectrometry, Lab Chip, 5, 1008-1016, 2005. 

Mass Spectrometry on a Chip Stationary Phases in Microchannels... 

Mass Spectrometry on a Chip, Fig. 1 (a) Emission characteristic of a microfabricated filament (Courtesy of Dr. Hyeun Joong Yoon) (b) emission characteristic of a silicon field emitter (Courtesy of Dr. Ejaz Huq) (c)... 

Mass Spectrometry on a Chip, Rgure 1 (d) Microfabricated plasma ion source with a travelling-wave mass filter (courtesy Prof. Jbrg Muller)... 

Mass Spectrometry on a Chip, Figure 3 (a) MIcrofabrIcated Wien mass filter by (courtesy Dr. Carl Freidhoff) (b) MIcrofabrIcated Faraday cup array (courtesy Prof. Bruce Darling)... 

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