5, 5 dithiobis-2 nitrobenzoic acid

Savas, M.M., Petering, D.H. and Shaw, C.F. Ill (1991) On the rapid, monophasic reaction of the rabbit liver metaDothionein a-domain with 5,5 -dithiobis (2-nitrobenzoic acid) (DTNB). Inorganic Chemistry, 30, 581-583. 

Mann and Mitchell [58] described a simple colorimetric method for estimation of (-D)-penicillamine in plasma. Blood containing 2-50 pg of penicillamine was mixed with 0.1 M EDTA solution in tromethamine buffer solution. 0.1 mL of this solution was adjusted to pH 7.4 and centrifuged. To a portion of the plasma was added 3 M HCL, the mixture was freeze-dried, and a suspension of the residue in ethanol was centrifuged. The supernatant liquid was mixed with tromethamine buffer solution (pH 8.2) and 10 mM 5,5 -dithiobis-(2-nitrobenzoic acid) in phosphate buffer solution (pH 7.0), the mixture was shaken with ethyl ether, and the absorbance of the separated aqueous layer was measured at 412 nm. The mean recovery was 60% (four determinations), and the calibration graph was linear for the cited range. 

Colman, R.F. (1969) The role of sulfhydryl groups in the catalytic function of isocitrate dehydrogenase. I. Reaction with 5,5 -dithiobis(2-nitrobenzoic acid). Biochemistry 8, 888. 

Kuwata, K., Uebori, M., Yamada, K., and Yamazaki, Y. (1982) Liquid chromatographic determination of alkylthiols via derivatization with 5,5 -dithiobis(2-nitrobenzoic acid). Anal. Chem. 54, 1082-1087. 

Riddles, P.W., Blakeley, R.L., and Zcrncr, B. (1979) Ellman s reagent 5,5 -dithiobis(2-nitrobenzoic acid)— A reexamination. Anal. Biochem. 94, 75-81. 

Two aromatic systems were investigated for vesicle insertion 5,5 -dithiobis(2-nitrobenzoic acid) and p-nitrophenylacetate. 

Ellman s reagent 5,5 -dithiobis(2-nitrobenzoic acid DTNB) reacts quantitatively with - SH groups (Eq. 3-41) to form mixed disulfides with release of a thiolate anion that absorbs light at 412 nm with a molar extinction... 

Similarly, Ellman s reagent [5,5 -dithiobis(2-nitrobenzoic acid)] can be used to activate one thiol-containing molecule by disulfide exchange and subsequently used to couple to a second sulfhydryl-containing molecule by the same mechanism (Chapter 1, Section 5.2) (Fig. 325). The disulfide of Ellman s reagent readily undergoes disulfide... 

Method A. The rationale of method A is that HDL and LDL are separated by selective precipitation of LDL by dextran sulfate and Mg2+ after the reaction between LDL and reconstituted HDL containing radiolabeled CE by CETP. The method was originally described by Kato et al. [77], The assay mixtures consist of reconstituted [14C]CE-HDL as the donor for CE, LDL as the acceptor, 5,5 -dithiobis-2-nitrobenzoic acid, bovine serum albumin (BSA), partially purified CETP, and a test sample in Eppendorf tubes (1.5 ml). After a 30-min incubation at 37°C, the reaction is terminated by the addition of an LDL-precipitation solution. After standing for 20 min in an ice bath, the assay mixtures are centrifuged, and the supernatant solution containing [14C]CE-HDL is analyzed for radioactivity. Furthermore, the [14C]CE-LDL precipitate is also analyzed for radioactivity if necessary. Usually the blank and control transfer values are about 6% and 34% of initial [14C]CE-HDL added under the assay conditions, respectively. 

The method is based on the partitions of the anions to a cationic micelle phase and shows different selectivity from ion exchange chromatography In a kinetic method [13] for the determination of thiocyanate, sulphite and sulphide the anions are reacted with 5,5 dithiobis (2-nitrobenzoic acid) in aqueous cetyltrimethyl/ammonium bromide micelles. 

Abbreviations used TNBS = 2,4,6-trinitrobenzenesulfonic acid DTNB = 5,5 -dithiobis-(2-nitrobenzoic acid) 2-PDS = 2,2 -dipyridyl disulfide 4-PDS = 4,4 -dipyridyl disulfide CMB = p-chloromercuribenzoic acid. 

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