5,5’ -Dithiobis-

The rates are essentially independent of the distribution of metal in the MT with similar rates between Zn7—MTm (Cd, Zn)7-MT, and Cd7—MT. The values of the rate constants are ks = 6.9( 0.9) x 10 " s and kf=2.7( 1.2) x 10 s for the holo-protein. The slow rate constant is similar in magnitude to the first-order protein-dependent steps observed for reactions of DTNB (5,5 -dithiobis(2-nitrobenzoate)), EDTA (ethylenediamine tetraacetate), cisplatin, and other reagents, which has been attributed to a rearrangement of the protein. The fast step is more rapid by an order of magnitude, which suggests that other mechanisms are prevailing. 

Savas, M.M., Petering, D.H. and Shaw, C.F. Ill (1991) On the rapid, monophasic reaction of the rabbit liver metaDothionein a-domain with 5,5 -dithiobis (2-nitrobenzoic acid) (DTNB). Inorganic Chemistry, 30, 581-583. 

Mann and Mitchell [58] described a simple colorimetric method for estimation of (-D)-penicillamine in plasma. Blood containing 2-50 pg of penicillamine was mixed with 0.1 M EDTA solution in tromethamine buffer solution. 0.1 mL of this solution was adjusted to pH 7.4 and centrifuged. To a portion of the plasma was added 3 M HCL, the mixture was freeze-dried, and a suspension of the residue in ethanol was centrifuged. The supernatant liquid was mixed with tromethamine buffer solution (pH 8.2) and 10 mM 5,5 -dithiobis-(2-nitrobenzoic acid) in phosphate buffer solution (pH 7.0), the mixture was shaken with ethyl ether, and the absorbance of the separated aqueous layer was measured at 412 nm. The mean recovery was 60% (four determinations), and the calibration graph was linear for the cited range. 

Colman, R.F. (1969) The role of sulfhydryl groups in the catalytic function of isocitrate dehydrogenase. I. Reaction with 5,5 -dithiobis(2-nitrobenzoic acid). Biochemistry 8, 888. 

Kuwata, K., Uebori, M., Yamada, K., and Yamazaki, Y. (1982) Liquid chromatographic determination of alkylthiols via derivatization with 5,5 -dithiobis(2-nitrobenzoic acid). Anal. Chem. 54, 1082-1087. 

Riddles, P.W., Blakeley, R.L., and Zcrncr, B. (1979) Ellman s reagent 5,5 -dithiobis(2-nitrobenzoic acid)— A reexamination. Anal. Biochem. 94, 75-81. 

Two aromatic systems were investigated for vesicle insertion 5,5 -dithiobis(2-nitrobenzoic acid) and p-nitrophenylacetate. 

Micelles in the Physical Chemistry Laboratory. Kinetics of Hydrolysis of 5,5 -Dithiobis-(2-nitrobenzoate) 246... 

Yang, Y. Zhou, H.-M. Reactivation kinetics of 5,5 -dithiobis-(2-nitroben-zoic acid)-modified creatine kinase reactivated by dithiothreitol. Biochim. Biophys. Acta, 1388, 190-198 (1998)... 

Ellman s reagent 5,5 -dithiobis(2-nitrobenzoic acid DTNB) reacts quantitatively with - SH groups (Eq. 3-41) to form mixed disulfides with release of a thiolate anion that absorbs light at 412 nm with a molar extinction... 

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