5,5’ -Dithiobis- acid

Savas, M.M., Petering, D.H. and Shaw, C.F. Ill (1991) On the rapid, monophasic reaction of the rabbit liver metaDothionein a-domain with 5,5 -dithiobis (2-nitrobenzoic acid) (DTNB). Inorganic Chemistry, 30, 581-583. 

Mann and Mitchell [58] described a simple colorimetric method for estimation of (-D)-penicillamine in plasma. Blood containing 2-50 pg of penicillamine was mixed with 0.1 M EDTA solution in tromethamine buffer solution. 0.1 mL of this solution was adjusted to pH 7.4 and centrifuged. To a portion of the plasma was added 3 M HCL, the mixture was freeze-dried, and a suspension of the residue in ethanol was centrifuged. The supernatant liquid was mixed with tromethamine buffer solution (pH 8.2) and 10 mM 5,5 -dithiobis-(2-nitrobenzoic acid) in phosphate buffer solution (pH 7.0), the mixture was shaken with ethyl ether, and the absorbance of the separated aqueous layer was measured at 412 nm. The mean recovery was 60% (four determinations), and the calibration graph was linear for the cited range. 

Figure 3.89 Cyclic voltammograms of 500 pm cytochrome c at a gold electrode modified by (a) 2-mercaptopyridine, (b> 2-mercaptosuccinic acid, 4,4 -dithiobis(butanoic acid), (d) 4-mercaploaniline. pH 7.0 phosphate buffer +0.1 M NaC104. Scan rale 50mVs . From Allen...

Colman, R.F. (1969) The role of sulfhydryl groups in the catalytic function of isocitrate dehydrogenase. I. Reaction with 5,5 -dithiobis(2-nitrobenzoic acid). Biochemistry 8, 888. 

Kuwata, K., Uebori, M., Yamada, K., and Yamazaki, Y. (1982) Liquid chromatographic determination of alkylthiols via derivatization with 5,5 -dithiobis(2-nitrobenzoic acid). Anal. Chem. 54, 1082-1087. 

Riddles, P.W., Blakeley, R.L., and Zcrncr, B. (1979) Ellman s reagent 5,5 -dithiobis(2-nitrobenzoic acid)— A reexamination. Anal. Biochem. 94, 75-81. 

Dipyridone, see Diquat Disacryl, see Acrolein Disulfoton oxon sulfone, see Disulfoton Disulfoton oxon sulfoxide, see Disulfoton Disulfoton sulfone, see Disulfoton Disulfoton sulfoxide, see Disulfoton 2,2 -Dithiobis-ethanol, see Phorate Dithiophosphoric acid, see Phorate... 

Two aromatic systems were investigated for vesicle insertion 5,5 -dithiobis(2-nitrobenzoic acid) and p-nitrophenylacetate. 

Dithiobis-(2-nitorobenzoic) acid (DTNB) as an LCAT inhibitor. 

Yang, Y. Zhou, H.-M. Reactivation kinetics of 5,5 -dithiobis-(2-nitroben-zoic acid)-modified creatine kinase reactivated by dithiothreitol. Biochim. Biophys. Acta, 1388, 190-198 (1998)... 

Ellman s reagent 5,5 -dithiobis(2-nitrobenzoic acid DTNB) reacts quantitatively with - SH groups (Eq. 3-41) to form mixed disulfides with release of a thiolate anion that absorbs light at 412 nm with a molar extinction... 

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